Q3A9N9 (SYE_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CHY_2340

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237350
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	249 – 253	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	252	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3A9N9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 28031AD0D5770BB3	FASTA	480	54,759
	10 20 30 40 50 60 MVRVRFAPSP TGPLHIGGAR SALFNYLFAR KNNGVFIVRI EDTDLERSSR ESEKNILESL 70 80 90 100 110 120 KWLGITWDEG IEVGGENGPY RQTERLDLYQ KYAQKLIEEG FAYYCFCTEE ELEEERKNLL 130 140 150 160 170 180 AKGEMPRYLG KCRNLTPEQK EKYLAEGRKP TVRFKVPAGR TIVINDLVRG VVSFETDGIG 190 200 210 220 230 240 DFIIVKSDGI PTYNFAVVID DVTMGITHVL RGEEHLSNTP RQILIYEALG FKIPEFAHIS 250 260 270 280 290 300 LILGKDRTKM SKRHGATSVE NYREKGYLPE ALVNFLALLG WSPGTEEEIF TMEQLIERFS 310 320 330 340 350 360 LDRVAKNPAI FDLDKLNWIN GYYIRNSELS RIVELSLPFF QSCGYVSQNP TEEEMRKLTK 370 380 390 400 410 420 VVEATREYVV TLSELPEHAA IFYQKELAFE EEAKTLLADE EARNILRKVA DKLREIPGSE 430 440 450 460 470 480 EEEIKGFLKK LPKELGVGGK KVYMPLRAAL TGKTHGPELY QVIAILGPAE AERRIMNLFN

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB13998.1.
RefSeq	YP_361145.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3A9N9.
SMR	Q3A9N9. Positions 1-477.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3A9N9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3726554.
GenomeReviews	Gene locus CHY_2340 in contig CP000141_GR.
KEGG	chy:CHY_2340.
NMPDR	fig|246194.3.peg.2506.
PATRIC	21277747. VBICarHyd26463_2226.
TIGR	CHY_2340.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	ESIIQFV.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_2340-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CARHZ

Accession

Primary (citable) accession number: Q3A9N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program