Q3A9N7 (ISPD_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	CHY_2342

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000237781
Sites
	Site	16	1	Transition state stabilizer By similarity
	Site	23	1	Transition state stabilizer By similarity
	Site	154	1	Positions MEP for the nucleophilic attack By similarity
	Site	210	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3A9N7 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 056584DD41DDC143	FASTA	233	25,501
	10 20 30 40 50 60 MEKIGVIVTA GGAGRRFGGE KPKQFLELSG IPVIIITLKR MVNLPIVGQV VLTVPENYIE 70 80 90 100 110 120 VAGDLLSRYN LAGIKLTAGG TTRRESVLRG IRALTGNFSI IAVHDGVRPF FPKGALSEGV 130 140 150 160 170 180 KKLSEGYGGA ILAVPLRDTV KEVKDNVVIS TLDRSRLYAV QTPQIFRREA LLKGHALGEK 190 200 210 220 230 QHLDAVDDSI LVELCGETVA VIPGDYKNLK ITWPEDLEFA EFLFTRYFAK ELL

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB14750.1.
RefSeq	YP_361147.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3A9N7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3A9N7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3728481.
GenomeReviews	Gene locus CHY_2342 in contig CP000141_GR.
KEGG	chy:CHY_2342.
NMPDR	fig|246194.3.peg.2508.
PATRIC	21277751. VBICarHyd26463_2228.
TIGR	CHY_2342.
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_2342-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_CARHZ

Accession

Primary (citable) accession number: Q3A9N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program