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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 151Transition state stabilizerUniRule annotation
Sitei22 – 221Transition state stabilizerUniRule annotation
Sitei155 – 1551Positions MEP for the nucleophilic attackUniRule annotation
Sitei211 – 2111Positions MEP for the nucleophilic attackUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciPCAR338963:GKDU-109-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:Pcar_0103
OrganismiPelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1)
Taxonomic identifieri338963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000002534 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2332332-C-methyl-D-erythritol 4-phosphate cytidylyltransferasePRO_0000237803Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi338963.Pcar_0103.

Structurei

3D structure databases

ProteinModelPortaliQ3A8C6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108EE7. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiQAYTPQM.
OrthoDBiPOG091H066R.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3A8C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVYVLIPAA GMGRRMGASV NKQYLPLGDR PVLSHTIALF DRHPGIDHIF
60 70 80 90 100
VICPEAEFPL CRREVIDPYD FRKVRGLVAG GAERQDSVRN GLQACQAAEG
110 120 130 140 150
DIVLIHDGAR PLLPPALIEP TVAAAQQCGA AIVGVPVKDT IKLVTDGMID
160 170 180 190 200
STPDRSLLWQ AQTPQAFRFG LIRDAHAQAL QQHHKGTDDA SLIEWLGGRV
210 220 230
AMIEGSYRNI KITTPEDLVL AQAFLDTPGD FQA
Length:233
Mass (Da):25,307
Last modified:November 22, 2005 - v1
Checksum:iD7057D8EC2BC572F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000142 Genomic DNA. Translation: ABA87366.1.
RefSeqiWP_011339755.1. NC_007498.2.

Genome annotation databases

EnsemblBacteriaiABA87366; ABA87366; Pcar_0103.
KEGGipca:Pcar_0103.
PATRICi22886058. VBIPelCar86875_0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000142 Genomic DNA. Translation: ABA87366.1.
RefSeqiWP_011339755.1. NC_007498.2.

3D structure databases

ProteinModelPortaliQ3A8C6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338963.Pcar_0103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA87366; ABA87366; Pcar_0103.
KEGGipca:Pcar_0103.
PATRICi22886058. VBIPelCar86875_0114.

Phylogenomic databases

eggNOGiENOG4108EE7. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiQAYTPQM.
OrthoDBiPOG091H066R.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciPCAR338963:GKDU-109-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPD_PELCD
AccessioniPrimary (citable) accession number: Q3A8C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 22, 2005
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.