Reviewed, UniProtKB/Swiss-Prot Q3A825 (NUBCD_PELCD)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B/C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B/C/D
    NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	Pcar_0206

Organism

Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) [Complete proteome] [HAMAP]

Taxonomic identifier

338963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Pelobacteraceae › Pelobacter

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits nuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358652
Regions
	Region	1 – 154	154	NADH dehydrogenase I subunit B By similarity
	Region	230 – 383	154	NADH dehydrogenase I subunit C By similarity
	Region	414 – 794	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3A825-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 61FB467B9AC50FC4	FASTA	794	88,844
	10 20 30 40 50 60 MSESIPKNII LTTLDDAINW GRKNSMWPMF FGLSCCFVEM MGSMTPRFDL ARFGAEVLRG 70 80 90 100 110 120 TPREADLMVI AGTPFKKMAA SMLRVYEEMA NPKWVIAMGS CANSGGMYDV YSVMQGVNQV 130 140 150 160 170 180 LPVDVYIPGC PPRPEAFMQG LMTLQEKIAR KESPARPVLN LRGGNEGTTV PILIDGSSKT 190 200 210 220 230 240 RDTRGPGYGH CPIRGTAQQH PDFKGHRAAG MWGPPQPKIA FSASTAPLLE QLRERFGDDV 250 260 270 280 290 300 RQEEGADMPT LVADPAQVPE LLRFLKQDID QPYERLEDLT AIDETARSQR PAHDLTMVYH 310 320 330 340 350 360 LLSFERAGYL RVKAPLRNDR AEVPSITGLW PAANWYEREA FDMFGVRFAG HPDLRRILMP 370 380 390 400 410 420 EGWQGHPLRK SHPSRATDMP PFTRDTATTM VPPEGSHFFS NRAPVDNQSL SILNLGPQHP 430 440 450 460 470 480 GTHGILRVIL QLAGEEIVDL DSEIGYHHRG AEKIAERQHW NQYIPYTDRI DYLAGSLNNL 490 500 510 520 530 540 AYLYSVETLC GVEVPPRARY IRVLLSELFR IASHLVWLGT FAHDVGAMTP VFYAFDSREK 550 560 570 580 590 600 IFDIIELITG GRMHPAWFRI GGVPDDLPDG WLAAVEAFTR DFEARIDEFD RLLTGGPIFR 610 620 630 640 650 660 ARTEGIGTVS RQQAVELGFS GPNLRATGLA WDLRKSMPYG GYDEFDFDVV TAGGGDCFAR 670 680 690 700 710 720 YLVRLGELRQ SLRIVRQAAE GMPQGRWISD QYRYAYPKRE DGLQDIESLI HHFLHVSRGP 730 740 750 760 770 780 AAPRGECYRA IESSKGECGY YAVSDGGTGA YRLRIRTPSF PHLQALPQLS RGRLVADVIT 790 ILGSLDYVLA DLDR

References

[1]

"Complete sequence of Pelobacter carbinolicus DSM 2380." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000142 Genomic DNA. Translation: ABA87467.1.
RefSeq	YP_355637.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3A825.
Genome annotation databases
GeneID	3723025.
GenomeReviews	Gene locus Pcar_0206 in contig CP000142_GR.
KEGG	pca:Pcar_0206.
NMPDR	fig|338963.3.peg.411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3A825.
OMA	PPTWEGH.
Enzyme and pathway databases
BioCyc	PCAR338963:PCAR_0206-MON.
Family and domain databases
HAMAP	MF_01356. Fused. [Tree] MF_01359. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit. TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_PELCD

Accession

Primary (citable) accession number: Q3A825

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)