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Q3A6I7 (TRMB_PELCD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Pcar_0761
OrganismPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) [Complete proteome] [HAMAP]
Taxonomic identifier338963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229181

Sites

Active site1131 By similarity
Binding site381S-adenosyl-L-methionine By similarity
Binding site631S-adenosyl-L-methionine By similarity
Binding site901S-adenosyl-L-methionine By similarity
Binding site1131S-adenosyl-L-methionine By similarity
Binding site1171Substrate By similarity
Binding site1491Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3A6I7 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 4A10A5DE1F337A22

FASTA23226,466
        10         20         30         40         50         60 
MTQHMIEIKS PYFLREESLA SPADFAAVFG NRNPLVLEIG CGIGDFVTQL ASSAPERNYL 

        70         80         90        100        110        120 
AIDIYNKGCN KTCNRLEQAR LTNVRVMRIE ARYLLEQFLA TESLAAVYIN CPDPWPKKRH 

       130        140        150        160        170        180 
LKRRLVNERF LQTLLHYLQP GGELFFSSDV ADYAHAVTAH LNQIKGYENR LPVPVALDMA 

       190        200        210        220        230 
GYPLSKYMRR FLAQGQSIHF IHHRRNPQHT CETAAPQPVH RGFRTAWSAN NG 

« Hide

References

[1]"Complete sequence of Pelobacter carbinolicus DSM 2380."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2380 / Gra Bd 1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000142 Genomic DNA. Translation: ABA88020.1.
RefSeqYP_006716458.1. NC_007498.2.

3D structure databases

ProteinModelPortalQ3A6I7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338963.Pcar_0761.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA88020; ABA88020; Pcar_0761.
GeneID3723255.
KEGGpca:Pcar_0761.
PATRIC22887520. VBIPelCar86875_0828.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000134942.
KOK03439.
OMAAEWIASH.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK827898.

Enzyme and pathway databases

BioCycPCAR338963:GKDU-857-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PELCD
AccessionPrimary (citable) accession number: Q3A6I7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways