Q3A4Q8 (RNC_PELCD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Pcar_1403

Organism

Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) [Complete proteome] [HAMAP]

Taxonomic identifier

338963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Pelobacteraceae › Pelobacter › Pelobacter carbinolicus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000228561
Regions
	Domain	7 – 136	130	RNase III
	Domain	163 – 232	70	DRBM
Sites
	Active site	53	1	Potential
	Active site	125	1	By similarity
	Metal binding	49	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Metal binding	125	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3A4Q8 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 816EC904967B5E58	FASTA	232	25,850
	10 20 30 40 50 60 MLEAKSAALL EDTIDYVFDN QALLLEALTH KSFSNEQNDR TEPDNERLEF LGDAVLGVVV 70 80 90 100 110 120 SHYIFRTFPH LPEGELTRIR SEVVSEKGLT VIGKAICLGD YMRLGKGEER SGGRQKSSLL 130 140 150 160 170 180 ANTMEALLGA VFCDGGFDSV RRVIEALFIP HIQRAARRKT GVDYKTRLQE RLQARFGDVP 190 200 210 220 230 QYVLIHADGP PHQRSYSVEA HFRGSCIGQG QGRSKKSAEQ AAAKQALEYL EE

References

[1]

"Complete sequence of Pelobacter carbinolicus DSM 2380." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2380 / Gra Bd 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000142 Genomic DNA. Translation: ABA88649.1.
RefSeq	YP_006717129.1. NC_007498.2.
3D structure databases
ProteinModelPortal	Q3A4Q8.
ModBase	Search...
Protein-protein interaction databases
STRING	338963.Pcar_1403.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA88649; ABA88649; Pcar_1403.
GeneID	3723041.
KEGG	pca:Pcar_1403.
PATRIC	22888942. VBIPelCar86875_1539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_PELCD

Accession

Primary (citable) accession number: Q3A4Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program