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Q3A4P8 (SYL_PELCD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pcar_1413
OrganismPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) [Complete proteome] [HAMAP]
Taxonomic identifier338963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter

Protein attributes

Sequence length851 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 851851Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009387

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif612 – 6165"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6151ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3A4P8 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 4066676129891262

FASTA85196,220
        10         20         30         40         50         60 
MEERYDAGGI EGKWQKRWEQ DRTFKAKEKD AREKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFKRLQG YNVLHPMGWD AFGMPAENAA IQHKTHPGKW TYENIDNMRA QLKKMGFSYD 

       130        140        150        160        170        180 
WDREVATCHP EYYRWEQLIF LKMLEKGLAY KKSSFVNWCS ECQTVLANEQ VENDACWRCG 

       190        200        210        220        230        240 
TVVEQKELEQ WFFKITDYAQ ELLDDTSKLS GWPEPVLTMQ RNWIGRSTGC EIDFPVADSD 

       250        260        270        280        290        300 
LKIKVFTTRQ DTLYGATFMS LAAEHPLAME LVSAECKAEV EAFIARVRTQ DKAKRTSDDF 

       310        320        330        340        350        360 
VKEGVFTGSY GINPVNGRRI PIYLANFVLM DYGTGAVMAV PTHDQRDFDF ARKYDIPMIV 

       370        380        390        400        410        420 
VIQPEDQTLD PATMTEAWTA PGVLVNSAPF DGLDNESAKE KIAAHLEEQG VGRKTVNYRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGTPIPIIYC PACGIVPVPE EDLPVLLPMD VEISGEGGSP LARHQEFLKV 

       490        500        510        520        530        540 
TCPTCGGDAR RETDTFDTFV ESSWYFARYT CPRFEQGPVD RATVDHWLPV DQYIGGIEHA 

       550        560        570        580        590        600 
VMHLLYARFF TKVMRDLGMM AHDEPFKNLL TQGMVCMETA SCPEHGWLFP EQVVEGKCVL 

       610        620        630        640        650        660 
CGATAQTGRN EKMSKSKKNV VDPDKLILSY GADTARLFSL FAAPPEKDLE WNEQGVEGCY 

       670        680        690        700        710        720 
RFLHRVWRAV YDNLEMISGV AVPASVDGAA RNLRRQVHAT IKKVTGDIDG RFHFNTAIAS 

       730        740        750        760        770        780 
VMELVNAIYG FEAKAQYPGV MREALEATVR LLAPFVPHVC EELWACLGHE GGLEAAGWPA 

       790        800        810        820        830        840 
WDEEALVEDE KTIVVQVNGK VRGKLTIAAD ADNDTVQQAA LKADNVVRFL EGKTVRKVVV 

       850 
VPGRLVNIVV S 

« Hide

References

[1]"Complete sequence of Pelobacter carbinolicus DSM 2380."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2380 / Gra Bd 1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000142 Genomic DNA. Translation: ABA88659.1.
RefSeqYP_006717139.1. NC_007498.2.

3D structure databases

ProteinModelPortalQ3A4P8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338963.Pcar_1413.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA88659; ABA88659; Pcar_1413.
GeneID3723839.
KEGGpca:Pcar_1413.
PATRIC22888964. VBIPelCar86875_1550.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPCAR338963:GKDU-1573-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PELCD
AccessionPrimary (citable) accession number: Q3A4P8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries