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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk-1

Organism
Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfk-1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
Metal bindingi118 – 1181Magnesium; catalyticUniRule annotation
Sitei119 – 1191Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei142 – 1421Proton acceptorUniRule annotation
Binding sitei177 – 1771Substrate; shared with dimeric partnerUniRule annotation
Binding sitei237 – 2371SubstrateUniRule annotation
Binding sitei281 – 2811Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi117 – 1204ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPCAR338963:GKDU-1673-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfk-1Imported
Synonyms:pfkAUniRule annotation
Ordered Locus Names:Pcar_1508Imported
OrganismiPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)Imported
Taxonomic identifieri338963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000002534 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi338963.Pcar_1508.

Structurei

3D structure databases

ProteinModelPortaliQ3A4F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 313309PFKInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1423Substrate bindingUniRule annotation
Regioni184 – 1863Substrate bindingUniRule annotation
Regioni287 – 2904Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3A4F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRCIALLNG GGDCPGLNGV IRGVVRAAVL QRGWRVLGVE DGFDGLVEGP
60 70 80 90 100
RCRELDLKSV CGILPRGGTI LGTSNRGNPF RYPVANENGD VILKDVSRQV
110 120 130 140 150
VDHFQKLGAD ALVAVGGDGT LKIARRLSDL GIPVVGVPKT IDNDLQETDV
160 170 180 190 200
TFGYNTAVGI VTEALDRLQT TAESHHRVMV VEVMGRDAGW IALEAGIAGS
210 220 230 240 250
AEVILLPEIP FDLDLVCDHI MDRRARGSRF SIIVVAEGAF PKGGRKIVQA
260 270 280 290 300
SADQTHGLER LGGIGHYVAA EIGRRKGLET RVVVLGHVQR GGTPSPFDRI
310 320 330 340 350
LASRFGVRAV QLIEREEYGH MVALRGRDVV SVPIEQAVDG QNLVDPDANL
360
VWTAEQLGIM LGR
Length:363
Mass (Da):38,904
Last modified:November 22, 2005 - v1
Checksum:i40DF99FCD29A3473
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000142 Genomic DNA. Translation: ABA88754.1.
RefSeqiWP_011341237.1. NC_007498.2.

Genome annotation databases

EnsemblBacteriaiABA88754; ABA88754; Pcar_1508.
KEGGipca:Pcar_1508.
PATRICi22889172. VBIPelCar86875_1649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000142 Genomic DNA. Translation: ABA88754.1.
RefSeqiWP_011341237.1. NC_007498.2.

3D structure databases

ProteinModelPortaliQ3A4F3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338963.Pcar_1508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA88754; ABA88754; Pcar_1508.
KEGGipca:Pcar_1508.
PATRICi22889172. VBIPelCar86875_1649.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciPCAR338963:GKDU-1673-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Pelobacter carbinolicus DSM 2380."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2380 / Gra Bd 1Imported.

Entry informationi

Entry nameiQ3A4F3_PELCD
AccessioniPrimary (citable) accession number: Q3A4F3
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.