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Q3A1R3

- SYI_PELCD

UniProt

Q3A1R3 - SYI_PELCD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPCAR338963:GKDU-2703-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Pcar_2455
    OrganismiPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
    Taxonomic identifieri338963 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter
    ProteomesiUP000002534: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 927927Isoleucine--tRNA ligasePRO_0000098436Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi338963.Pcar_2455.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3A1R3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3A1R3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKQTLNLP DTQFPMRGNL PKREPEILEK WQSMGLYEKM EEAGRTRPNF    50
    TLHDGPPYAN GHIHIGHALN KILKDIVLKS RRMKGFYAPY VPGWDCHGLP 100
    IELMVDKKLG KKKRDMTKVE IRKECRVYAD QWVKIQSEEF ERLGVMGEWD 150
    RPYLTMTHHY EAVTARELAR FVEKGGLFKG KKPIHWCSSC VTALAEAEVE 200
    YADHKSPSIY VKFPFDGELP AELNDLAGRK LSFVIWTTTP WTIPANLAVC 250
    LNPNLPYAVV ETGDELLVMA EGLVSGVMQE LGLENYRVLK TFEAPIFERK 300
    TCRHPFYDRP SLLILGDHVT LEAGTGCVHT APGHGQDDYV VGLAYGLDVY 350
    NPVDNYGRYY EDVEFFGGMK INQANGAVNA KLTEVGALLK ESEVSHSYPH 400
    CWRCKKPIIF RATEQWFISM EANGLREKAL GHINDVNWVP RWGRDRIYNM 450
    VENRPDWCIS RQRSWGVPIT IFYCAKCGEA LEDSKVMDYV ADLFEEGGSD 500
    LWFDKPAKEL MPAGTTCPGC GHDEFTKETD ILDVWFDSGV SHAAVLDNRD 550
    YLSWPADLYL EGSDQHRGWF HSSLLASVGT RELTPYKSVL THGFVVDGKG 600
    KKMSKSVGNV VAPEEVIKKY GAEILRLWVA AQDYRDDIRI SNEILQRLSD 650
    AYRRIRNTAR YILGNLSGFD PSRDMVADDQ LLELDRWALA KLEDLAGRVE 700
    KAYEDYEFHI IYHAVHNFCS VEMSSFYLDV LKDRLYVSGT DSIARRSAQT 750
    AMYRILDCIT RLIAPVLSFT AEEIWAFMPG ERSESVHLGE FVQFPTSFRD 800
    AALEERYDNL LEIRSDVSKA LELARNEKVI GHSLDARVLL SAPAGATLEL 850
    LEKYRDQLAS LFIVSQVELV DDLADGLTGE NLPDLKVKVE KALGEKCERC 900
    WNYATSVGDS AEHPALCHRC VAVLTDR 927
    Length:927
    Mass (Da):105,258
    Last modified:November 22, 2005 - v1
    Checksum:i835B83A6EE0B9CF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000142 Genomic DNA. Translation: ABA89694.1.
    RefSeqiYP_006718218.1. NC_007498.2.

    Genome annotation databases

    EnsemblBacteriaiABA89694; ABA89694; Pcar_2455.
    GeneIDi3725157.
    KEGGipca:Pcar_2455.
    PATRICi22891226. VBIPelCar86875_2653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000142 Genomic DNA. Translation: ABA89694.1 .
    RefSeqi YP_006718218.1. NC_007498.2.

    3D structure databases

    ProteinModelPortali Q3A1R3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 338963.Pcar_2455.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA89694 ; ABA89694 ; Pcar_2455 .
    GeneIDi 3725157.
    KEGGi pca:Pcar_2455.
    PATRICi 22891226. VBIPelCar86875_2653.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PCAR338963:GKDU-2703-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Pelobacter carbinolicus DSM 2380."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2380 / Gra Bd 1.

    Entry informationi

    Entry nameiSYI_PELCD
    AccessioniPrimary (citable) accession number: Q3A1R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3