Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q3A1R3

- SYI_PELCD

UniProt

Q3A1R3 - SYI_PELCD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPCAR338963:GKDU-2703-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Pcar_2455
OrganismiPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Taxonomic identifieri338963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter
ProteomesiUP000002534: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligasePRO_0000098436Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi338963.Pcar_2455.

Structurei

3D structure databases

ProteinModelPortaliQ3A1R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3A1R3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKQTLNLP DTQFPMRGNL PKREPEILEK WQSMGLYEKM EEAGRTRPNF
60 70 80 90 100
TLHDGPPYAN GHIHIGHALN KILKDIVLKS RRMKGFYAPY VPGWDCHGLP
110 120 130 140 150
IELMVDKKLG KKKRDMTKVE IRKECRVYAD QWVKIQSEEF ERLGVMGEWD
160 170 180 190 200
RPYLTMTHHY EAVTARELAR FVEKGGLFKG KKPIHWCSSC VTALAEAEVE
210 220 230 240 250
YADHKSPSIY VKFPFDGELP AELNDLAGRK LSFVIWTTTP WTIPANLAVC
260 270 280 290 300
LNPNLPYAVV ETGDELLVMA EGLVSGVMQE LGLENYRVLK TFEAPIFERK
310 320 330 340 350
TCRHPFYDRP SLLILGDHVT LEAGTGCVHT APGHGQDDYV VGLAYGLDVY
360 370 380 390 400
NPVDNYGRYY EDVEFFGGMK INQANGAVNA KLTEVGALLK ESEVSHSYPH
410 420 430 440 450
CWRCKKPIIF RATEQWFISM EANGLREKAL GHINDVNWVP RWGRDRIYNM
460 470 480 490 500
VENRPDWCIS RQRSWGVPIT IFYCAKCGEA LEDSKVMDYV ADLFEEGGSD
510 520 530 540 550
LWFDKPAKEL MPAGTTCPGC GHDEFTKETD ILDVWFDSGV SHAAVLDNRD
560 570 580 590 600
YLSWPADLYL EGSDQHRGWF HSSLLASVGT RELTPYKSVL THGFVVDGKG
610 620 630 640 650
KKMSKSVGNV VAPEEVIKKY GAEILRLWVA AQDYRDDIRI SNEILQRLSD
660 670 680 690 700
AYRRIRNTAR YILGNLSGFD PSRDMVADDQ LLELDRWALA KLEDLAGRVE
710 720 730 740 750
KAYEDYEFHI IYHAVHNFCS VEMSSFYLDV LKDRLYVSGT DSIARRSAQT
760 770 780 790 800
AMYRILDCIT RLIAPVLSFT AEEIWAFMPG ERSESVHLGE FVQFPTSFRD
810 820 830 840 850
AALEERYDNL LEIRSDVSKA LELARNEKVI GHSLDARVLL SAPAGATLEL
860 870 880 890 900
LEKYRDQLAS LFIVSQVELV DDLADGLTGE NLPDLKVKVE KALGEKCERC
910 920
WNYATSVGDS AEHPALCHRC VAVLTDR
Length:927
Mass (Da):105,258
Last modified:November 22, 2005 - v1
Checksum:i835B83A6EE0B9CF5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000142 Genomic DNA. Translation: ABA89694.1.
RefSeqiYP_006718218.1. NC_007498.2.

Genome annotation databases

EnsemblBacteriaiABA89694; ABA89694; Pcar_2455.
GeneIDi3725157.
KEGGipca:Pcar_2455.
PATRICi22891226. VBIPelCar86875_2653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000142 Genomic DNA. Translation: ABA89694.1 .
RefSeqi YP_006718218.1. NC_007498.2.

3D structure databases

ProteinModelPortali Q3A1R3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 338963.Pcar_2455.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA89694 ; ABA89694 ; Pcar_2455 .
GeneIDi 3725157.
KEGGi pca:Pcar_2455.
PATRICi 22891226. VBIPelCar86875_2653.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PCAR338963:GKDU-2703-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Pelobacter carbinolicus DSM 2380."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2380 / Gra Bd 1.

Entry informationi

Entry nameiSYI_PELCD
AccessioniPrimary (citable) accession number: Q3A1R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3