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Q3A1R3

- SYI_PELCD

UniProt

Q3A1R3 - SYI_PELCD

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Pcar_2455
Organism
Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPCAR338963:GKDU-2703-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Pcar_2455
OrganismiPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Taxonomic identifieri338963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter
ProteomesiUP000002534: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligaseUniRule annotationPRO_0000098436Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi338963.Pcar_2455.

Structurei

3D structure databases

ProteinModelPortaliQ3A1R3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3A1R3-1 [UniParc]FASTAAdd to Basket

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MDYKQTLNLP DTQFPMRGNL PKREPEILEK WQSMGLYEKM EEAGRTRPNF    50
TLHDGPPYAN GHIHIGHALN KILKDIVLKS RRMKGFYAPY VPGWDCHGLP 100
IELMVDKKLG KKKRDMTKVE IRKECRVYAD QWVKIQSEEF ERLGVMGEWD 150
RPYLTMTHHY EAVTARELAR FVEKGGLFKG KKPIHWCSSC VTALAEAEVE 200
YADHKSPSIY VKFPFDGELP AELNDLAGRK LSFVIWTTTP WTIPANLAVC 250
LNPNLPYAVV ETGDELLVMA EGLVSGVMQE LGLENYRVLK TFEAPIFERK 300
TCRHPFYDRP SLLILGDHVT LEAGTGCVHT APGHGQDDYV VGLAYGLDVY 350
NPVDNYGRYY EDVEFFGGMK INQANGAVNA KLTEVGALLK ESEVSHSYPH 400
CWRCKKPIIF RATEQWFISM EANGLREKAL GHINDVNWVP RWGRDRIYNM 450
VENRPDWCIS RQRSWGVPIT IFYCAKCGEA LEDSKVMDYV ADLFEEGGSD 500
LWFDKPAKEL MPAGTTCPGC GHDEFTKETD ILDVWFDSGV SHAAVLDNRD 550
YLSWPADLYL EGSDQHRGWF HSSLLASVGT RELTPYKSVL THGFVVDGKG 600
KKMSKSVGNV VAPEEVIKKY GAEILRLWVA AQDYRDDIRI SNEILQRLSD 650
AYRRIRNTAR YILGNLSGFD PSRDMVADDQ LLELDRWALA KLEDLAGRVE 700
KAYEDYEFHI IYHAVHNFCS VEMSSFYLDV LKDRLYVSGT DSIARRSAQT 750
AMYRILDCIT RLIAPVLSFT AEEIWAFMPG ERSESVHLGE FVQFPTSFRD 800
AALEERYDNL LEIRSDVSKA LELARNEKVI GHSLDARVLL SAPAGATLEL 850
LEKYRDQLAS LFIVSQVELV DDLADGLTGE NLPDLKVKVE KALGEKCERC 900
WNYATSVGDS AEHPALCHRC VAVLTDR 927
Length:927
Mass (Da):105,258
Last modified:November 22, 2005 - v1
Checksum:i835B83A6EE0B9CF5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000142 Genomic DNA. Translation: ABA89694.1.
RefSeqiYP_006718218.1. NC_007498.2.

Genome annotation databases

EnsemblBacteriaiABA89694; ABA89694; Pcar_2455.
GeneIDi3725157.
KEGGipca:Pcar_2455.
PATRICi22891226. VBIPelCar86875_2653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000142 Genomic DNA. Translation: ABA89694.1 .
RefSeqi YP_006718218.1. NC_007498.2.

3D structure databases

ProteinModelPortali Q3A1R3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 338963.Pcar_2455.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA89694 ; ABA89694 ; Pcar_2455 .
GeneIDi 3725157.
KEGGi pca:Pcar_2455.
PATRICi 22891226. VBIPelCar86875_2653.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PCAR338963:GKDU-2703-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Pelobacter carbinolicus DSM 2380."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chertkov O., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2380 / Gra Bd 1.

Entry informationi

Entry nameiSYI_PELCD
AccessioniPrimary (citable) accession number: Q3A1R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi