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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi897ZincUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi917ZincUniRule annotation1
Metal bindingi920ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPCAR338963:G1G5C-2579-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Pcar_2455
OrganismiPelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1)
Taxonomic identifieri338963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000002534 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984361 – 927Isoleucine--tRNA ligaseAdd BLAST927

Proteomic databases

PRIDEiQ3A1R3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi338963.Pcar_2455

Structurei

3D structure databases

ProteinModelPortaliQ3A1R3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q3A1R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKQTLNLP DTQFPMRGNL PKREPEILEK WQSMGLYEKM EEAGRTRPNF
60 70 80 90 100
TLHDGPPYAN GHIHIGHALN KILKDIVLKS RRMKGFYAPY VPGWDCHGLP
110 120 130 140 150
IELMVDKKLG KKKRDMTKVE IRKECRVYAD QWVKIQSEEF ERLGVMGEWD
160 170 180 190 200
RPYLTMTHHY EAVTARELAR FVEKGGLFKG KKPIHWCSSC VTALAEAEVE
210 220 230 240 250
YADHKSPSIY VKFPFDGELP AELNDLAGRK LSFVIWTTTP WTIPANLAVC
260 270 280 290 300
LNPNLPYAVV ETGDELLVMA EGLVSGVMQE LGLENYRVLK TFEAPIFERK
310 320 330 340 350
TCRHPFYDRP SLLILGDHVT LEAGTGCVHT APGHGQDDYV VGLAYGLDVY
360 370 380 390 400
NPVDNYGRYY EDVEFFGGMK INQANGAVNA KLTEVGALLK ESEVSHSYPH
410 420 430 440 450
CWRCKKPIIF RATEQWFISM EANGLREKAL GHINDVNWVP RWGRDRIYNM
460 470 480 490 500
VENRPDWCIS RQRSWGVPIT IFYCAKCGEA LEDSKVMDYV ADLFEEGGSD
510 520 530 540 550
LWFDKPAKEL MPAGTTCPGC GHDEFTKETD ILDVWFDSGV SHAAVLDNRD
560 570 580 590 600
YLSWPADLYL EGSDQHRGWF HSSLLASVGT RELTPYKSVL THGFVVDGKG
610 620 630 640 650
KKMSKSVGNV VAPEEVIKKY GAEILRLWVA AQDYRDDIRI SNEILQRLSD
660 670 680 690 700
AYRRIRNTAR YILGNLSGFD PSRDMVADDQ LLELDRWALA KLEDLAGRVE
710 720 730 740 750
KAYEDYEFHI IYHAVHNFCS VEMSSFYLDV LKDRLYVSGT DSIARRSAQT
760 770 780 790 800
AMYRILDCIT RLIAPVLSFT AEEIWAFMPG ERSESVHLGE FVQFPTSFRD
810 820 830 840 850
AALEERYDNL LEIRSDVSKA LELARNEKVI GHSLDARVLL SAPAGATLEL
860 870 880 890 900
LEKYRDQLAS LFIVSQVELV DDLADGLTGE NLPDLKVKVE KALGEKCERC
910 920
WNYATSVGDS AEHPALCHRC VAVLTDR
Length:927
Mass (Da):105,258
Last modified:November 22, 2005 - v1
Checksum:i835B83A6EE0B9CF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000142 Genomic DNA Translation: ABA89694.1
RefSeqiWP_011342220.1, NC_007498.2

Genome annotation databases

EnsemblBacteriaiABA89694; ABA89694; Pcar_2455
KEGGipca:Pcar_2455

Similar proteinsi

Entry informationi

Entry nameiSYI_PELCD
AccessioniPrimary (citable) accession number: Q3A1R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health