Q39ZC4 (NUBCD_GEOMG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit B/C/D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit B/C/D
NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	Gmet_0153

Organism

Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]

Taxonomic identifier

269799 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01356
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits NuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_01356.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 791	791	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358643
Regions
	Region	1 – 155	155	NADH dehydrogenase I subunit B By similarity
	Region	229 – 384	156	NADH dehydrogenase I subunit C By similarity
	Region	411 – 791	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39ZC4 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 8E9C5038E36A6B71	FASTA	791	90,369
	10 20 30 40 50 60 MEETEIPQNI VLTRLDDLIN WGRANSLWPM FFGLSCCFVE MMTSFTSRYD ISRFGAEVLR 70 80 90 100 110 120 GTPREADLMV IAGTVFKKMA PSILRLYEQM AEPKWVISMG SCANSGGMYD VYSVVQGVNQ 130 140 150 160 170 180 IIPVDVHVPG CPPRPEAFLQ GVMLLQEKIR REERPARKVL HMAGGTEGTT RPVLVDGVTK 190 200 210 220 230 240 SRDTRGPGME GIAIRGTPVQ HPRFWMSRSD EMWRPPAPRH DYPDFGLAGE LETIFGGRVA 250 260 270 280 290 300 VEQAATDMLT YRCPPELVPE VLRHLKSRSS APFRRLEDVV CVDESCRRER ERFPDFTVNY 310 320 330 340 350 360 HLLNFDIPGH LRIKTELMGE TPELPSATGV FPAADWYERE AFDMYGIRFA GHPNLRRILM 370 380 390 400 410 420 PPDWEGHPLR KEHPFRATEM PPYTTDDARR HQALPASDFF DRIDEETLII NLGPQHPGTH 430 440 450 460 470 480 GIIRFILKLD GEEIVDMDTD IGYHHRGAEK IGERQHWNQF IPYTDRIDYL AGVQNNLAYV 490 500 510 520 530 540 NSVERLCGIT VPDRAITIRV MLAELFRIAS HLVWLGTFAA DVGAMTPVFY TFTDREKIFD 550 560 570 580 590 600 IIEMVTGGRM HPSWFRIGGV TEDLPEGWDG AVKEFLDWMP GRLKEYEDLL KENPIFKARL 610 620 630 640 650 660 KGVGVITKDE AMEWGITGPN LRACGMAWDL RKKIPYNGYQ HFHFEIPTEE GGDCWARYRI 670 680 690 700 710 720 RVEEIRQSLH IVAQCRKEMP AGRWITDDYR YVLPKKRDTL HDIESLIHHF INATRGMAPP 730 740 750 760 770 780 RGENYSAIEA PKGENGYFVV SDGLNVPYRV RIKTPSFPHI QALPLMSRGW LVADFLAIIG 790 SIDFVLADLD R

References

[1]

"Complete sequence of Geobacter metallireducens GS-15." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GS-15 / ATCC 53774 / DSM 7210.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000148 Genomic DNA. Translation: ABB30400.1.
RefSeq	YP_383125.1. NC_007517.1.
3D structure databases
HSSP	HSSP built from PDB template 2FUG based on UniProtKB Q56218.
ProteinModelPortal	Q39ZC4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39ZC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3739634.
GenomeReviews	Gene locus Gmet_0153 in contig CP000148_GR.
KEGG	gme:Gmet_0153.
NMPDR	fig|269799.3.peg.51.
PATRIC	21999442. VBIGeoMet55070_0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0649.
HOGENOM	HBG459705.
OMA	PHLQQIP.
PhylomeDB	Q39ZC4.
ProtClustDB	PRK13292.
Enzyme and pathway databases
BioCyc	GMET269799:GMET_0153-MONOMER.
Family and domain databases
HAMAP	MF_01356. NDH1_NuoB. Fused. [Tree] MF_01359. NDH1_NuoCD_1. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_suD. IPR010218. NADH_DH_suC. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR022885. NDH1_su_D/H. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K13380.
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_GEOMG

Accession

Primary (citable) accession number: Q39ZC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program