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Q39YT0

- Q39YT0_GEOMG

UniProt

Q39YT0 - Q39YT0_GEOMG

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Gmet_0351
Organism
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei604 – 6041ATP By similarityUniRule annotation
Metal bindingi895 – 8951Zinc By similarityUniRule annotation
Metal bindingi898 – 8981Zinc By similarityUniRule annotation
Metal bindingi915 – 9151Zinc By similarityUniRule annotation
Metal bindingi918 – 9181Zinc By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding, ZincUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciGMET269799:GHNY-351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:Gmet_0351Imported
OrganismiGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)Imported
Taxonomic identifieri269799 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000007073: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi269799.Gmet_0351.

Structurei

3D structure databases

ProteinModelPortaliQ39YT0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi601 – 6055"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39YT0-1 [UniParc]FASTAAdd to Basket

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MEYKNTLNLP VTDFPMKANL PQREPEILAG WQKNDLYGKL TAAGEGKPRY    50
VLHDGPPYAN GHIHIGHALN KILKDIILKS KRMQGFDAPY VPGWDCHGLP 100
IELQVEKNLG SKKHETTKLQ MRKQCREYAE KFVKIQRDEF ERLGVIGDWD 150
RPYLTMTHDY EGITARELAR FAANGGLYKG KKPVHWCSSC VTALAEAEVE 200
YADKTSPSIY VKFLLQDDIS ASVPALAGKK VSLVIWTTTP WTIPANLAVA 250
LHPELEYVAL EAGGEVLVVA EGLKDAFMAA TGVQGSVIAT FRADILYRKR 300
CKHPFYDRDS IVLLGEHVTL DAGTGCVHTA PGHGQEDYEL ALVEGLDIYN 350
PVDNRGRYIQ SLEFFGGQFV FDANATVIEK LQEVGALVGQ GSVEHSYPHC 400
WRCKKPIIFR ATEQWFISME KNDLRKKALE EIDRVSWVPK WGRERIHGMI 450
ENRPDWCISR QRSWGVPITA FYCTECGEIL ADGTIMHHVA DLFMEGGADL 500
WYEKEAAELL PPGTVCPQCG KSAFEKEMDI LDVWFDSGVS HAAVLENRPE 550
LGSPANMYLE GSDQHRGWFH SSLLASVGTR GTAPYKEVLT HGFVVDGSGR 600
KMSKSVGNVV APEEVIKKYG AEILRLWVAA QDYRDDVRIS QEILTRLAEA 650
YRRIRNTCRY LLGNLYDFDP ATDMVPFGEM TELDRWALHQ LEVLKEKVFT 700
AYNEYEFHIL YHAVNGFCTV EMSAFYLDII KERYTSRKDS PERRSAQTVM 750
YLVLESLVRL MAPVLSFTAD EVWGYMPKRA EASVHLASFP ELCPERKDEA 800
LVERWARTMA VRGDVSKALE QARVQKTIGH SLDAAVTLSA EPELLGFLKE 850
YAGELATVFI VSKVELVGEI AGEFVEAEGV KGLKIGVTAA PGDKCERCWH 900
YDEEIGGDAE HPTLCPKCVA AVK 923
Length:923
Mass (Da):103,598
Last modified:November 22, 2005 - v1
Checksum:i7BCF7E69BF1B2E7F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000148 Genomic DNA. Translation: ABB30594.1.
RefSeqiYP_006719314.1. NC_007517.1.

Genome annotation databases

EnsemblBacteriaiABB30594; ABB30594; Gmet_0351.
GeneIDi3739717.
KEGGigme:Gmet_0351.
PATRICi21999848. VBIGeoMet55070_0371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000148 Genomic DNA. Translation: ABB30594.1 .
RefSeqi YP_006719314.1. NC_007517.1.

3D structure databases

ProteinModelPortali Q39YT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269799.Gmet_0351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB30594 ; ABB30594 ; Gmet_0351 .
GeneIDi 3739717.
KEGGi gme:Gmet_0351.
PATRICi 21999848. VBIGeoMet55070_0371.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci GMET269799:GHNY-351-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Geobacter metallireducens GS-15."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS-15 / ATCC 53774 / DSM 7210.

Entry informationi

Entry nameiQ39YT0_GEOMG
AccessioniPrimary (citable) accession number: Q39YT0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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