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Q39YT0

- Q39YT0_GEOMG

UniProt

Q39YT0 - Q39YT0_GEOMG

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Note: Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
Binding sitei604 – 6041ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciGMET269799:GHNY-351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:Gmet_0351Imported
OrganismiGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)Imported
Taxonomic identifieri269799 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000007073: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi269799.Gmet_0351.

Structurei

3D structure databases

ProteinModelPortaliQ39YT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi601 – 6055"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39YT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEYKNTLNLP VTDFPMKANL PQREPEILAG WQKNDLYGKL TAAGEGKPRY
60 70 80 90 100
VLHDGPPYAN GHIHIGHALN KILKDIILKS KRMQGFDAPY VPGWDCHGLP
110 120 130 140 150
IELQVEKNLG SKKHETTKLQ MRKQCREYAE KFVKIQRDEF ERLGVIGDWD
160 170 180 190 200
RPYLTMTHDY EGITARELAR FAANGGLYKG KKPVHWCSSC VTALAEAEVE
210 220 230 240 250
YADKTSPSIY VKFLLQDDIS ASVPALAGKK VSLVIWTTTP WTIPANLAVA
260 270 280 290 300
LHPELEYVAL EAGGEVLVVA EGLKDAFMAA TGVQGSVIAT FRADILYRKR
310 320 330 340 350
CKHPFYDRDS IVLLGEHVTL DAGTGCVHTA PGHGQEDYEL ALVEGLDIYN
360 370 380 390 400
PVDNRGRYIQ SLEFFGGQFV FDANATVIEK LQEVGALVGQ GSVEHSYPHC
410 420 430 440 450
WRCKKPIIFR ATEQWFISME KNDLRKKALE EIDRVSWVPK WGRERIHGMI
460 470 480 490 500
ENRPDWCISR QRSWGVPITA FYCTECGEIL ADGTIMHHVA DLFMEGGADL
510 520 530 540 550
WYEKEAAELL PPGTVCPQCG KSAFEKEMDI LDVWFDSGVS HAAVLENRPE
560 570 580 590 600
LGSPANMYLE GSDQHRGWFH SSLLASVGTR GTAPYKEVLT HGFVVDGSGR
610 620 630 640 650
KMSKSVGNVV APEEVIKKYG AEILRLWVAA QDYRDDVRIS QEILTRLAEA
660 670 680 690 700
YRRIRNTCRY LLGNLYDFDP ATDMVPFGEM TELDRWALHQ LEVLKEKVFT
710 720 730 740 750
AYNEYEFHIL YHAVNGFCTV EMSAFYLDII KERYTSRKDS PERRSAQTVM
760 770 780 790 800
YLVLESLVRL MAPVLSFTAD EVWGYMPKRA EASVHLASFP ELCPERKDEA
810 820 830 840 850
LVERWARTMA VRGDVSKALE QARVQKTIGH SLDAAVTLSA EPELLGFLKE
860 870 880 890 900
YAGELATVFI VSKVELVGEI AGEFVEAEGV KGLKIGVTAA PGDKCERCWH
910 920
YDEEIGGDAE HPTLCPKCVA AVK
Length:923
Mass (Da):103,598
Last modified:November 22, 2005 - v1
Checksum:i7BCF7E69BF1B2E7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB30594.1.
RefSeqiWP_004512322.1. NC_007517.1.
YP_006719314.1. NC_007517.1.

Genome annotation databases

EnsemblBacteriaiABB30594; ABB30594; Gmet_0351.
GeneIDi3739717.
KEGGigme:Gmet_0351.
PATRICi21999848. VBIGeoMet55070_0371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB30594.1 .
RefSeqi WP_004512322.1. NC_007517.1.
YP_006719314.1. NC_007517.1.

3D structure databases

ProteinModelPortali Q39YT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269799.Gmet_0351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB30594 ; ABB30594 ; Gmet_0351 .
GeneIDi 3739717.
KEGGi gme:Gmet_0351.
PATRICi 21999848. VBIGeoMet55070_0371.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci GMET269799:GHNY-351-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Geobacter metallireducens GS-15."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS-15 / ATCC 53774 / DSM 7210Imported.

Entry informationi

Entry nameiQ39YT0_GEOMG
AccessioniPrimary (citable) accession number: Q39YT0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3