Reviewed, UniProtKB/Swiss-Prot Q39YM4 (MURE_GEOMG)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	Gmet_0407

Organism

Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]

Taxonomic identifier

269799 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012353
Regions
	Nucleotide binding	110 – 116	7	ATP Potential
	Region	152 – 153	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	409 – 412	4	Meso-diaminopimelate binding By similarity
	Motif	409 – 412	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	179	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	185	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	187	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	385	1	Meso-diaminopimelate By similarity
	Binding site	476	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	480	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	219	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39YM4-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 8700F06EAB4E3E6B	FASTA	511	54,733
	10 20 30 40 50 60 MRLEDLARVI NPSEAGGSLA LEIEGLYCDS RQVRSGGLFF ALRGVATDGH DYIVASRDRG 70 80 90 100 110 120 AVAVVVEDPS RVPEGMTWLK VDDARLAMSR AAAAFYGNPT DGIPVVGITG TNGKTTTTYL 130 140 150 160 170 180 VEAVMARAGI PVAVLGTISY RFGDKTIPAP HTTPESVDLQ RTIRDLVDRG AQGVVMEVSS 190 200 210 220 230 240 HALEQRRVDG CRFDVGVFTN LTRDHLDYHR DMESYFESKA RLFTELLAAD DTKPRRAAVI 250 260 270 280 290 300 NVDDPYGARL ASDAVAPVVS YGLSPSVMVR AEMVTFSVDG ISGTLVTPLG TIPFHSRLLG 310 320 330 340 350 360 RFNLYNILAA VAAGVALGLS PDAIRGGIEG DVRVPGRLER VDNDHGITVL VDYAHTGDAL 370 380 390 400 410 420 ENVLKTVAEI ATGRIITVFG CGGDRDRGKR PVMGEIAGRF SDLSIVTSDN PRTEDAGAII 430 440 450 460 470 480 GEILGGIRSL GLREYGAEEL ITGFDAKGFA AVESRREAIR LAARVARPGD VLLLAGKGHE 490 500 510 DYQIIGTMKH HFDDREEVAA AFRELSSKGQ G

References

[1]

"Complete sequence of Geobacter metallireducens GS-15." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000148 Genomic DNA. Translation: ABB30650.1.
RefSeq	YP_383375.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39YM4.
Genome annotation databases
GeneID	3738162.
GenomeReviews	Gene locus Gmet_0407 in contig CP000148_GR.
KEGG	gme:Gmet_0407.
NMPDR	fig|269799.3.peg.435.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39YM4.
OMA	FPVIVDY.
Enzyme and pathway databases
BioCyc	GMET269799:GMET_0407-MON.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_GEOMG

Accession

Primary (citable) accession number: Q39YM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)