Q39WQ9 (CHEB1_GEOMG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Gmet_1075

Organism

Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]

Taxonomic identifier

269799 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264276
Regions
	Domain	7 – 124	118	Response regulatory
	Domain	164 – 356	193	CheB-type methylesterase
Sites
	Active site	176	1	By similarity
	Active site	202	1	By similarity
	Active site	298	1	By similarity
Amino acid modifications
	Modified residue	58	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39WQ9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 5C8D858CA230EB40	FASTA	363	39,686
	10 20 30 40 50 60 MSTQPIKVLI VDDSALIRSL LTDIINSQTD MEVVGVAPDP LVAREKIKAL NPDVLTLDVE 70 80 90 100 110 120 MPRMDGLVFL EKLMRLRPMP VLMVSSLTEK SSFLTLRALE LGAVDFVTKP KIDISRGMQE 130 140 150 160 170 180 YAREITDKLR VAAKARVRQP PHVHLSVERK NTADAVLPME HRTFSSTEKI IVIGASTGGT 190 200 210 220 230 240 EALKSFLVAM PADSPGILIT QHMPEAFTRT FAQRLNSLCR ISVKEAEHGE RILPGHAYVA 250 260 270 280 290 300 PGNRHLLLAR SGANYVVELS DGPPVSRHRP SVDVLFRSAA NRAGRNAVGI IMTGMGDDGA 310 320 330 340 350 360 AGMLEMREAG AYTFAQDEKS CVVFGMPKEA IARGGVDEVA PLGEMPRRLF GWLESQGNRA FRV

References

[1]

"Complete sequence of Geobacter metallireducens GS-15." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GS-15 / ATCC 53774 / DSM 7210.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000148 Genomic DNA. Translation: ABB31315.1.
RefSeq	YP_384040.1. NC_007517.1.
3D structure databases
ProteinModelPortal	Q39WQ9.
SMR	Q39WQ9. Positions 6-351.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39WQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3741282.
GenomeReviews	Gene locus Gmet_1075 in contig CP000148_GR.
KEGG	gme:Gmet_1075.
NMPDR	fig|269799.3.peg.1459.
PATRIC	22001344. VBIGeoMet55070_1112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	FVTKPKL.
PhylomeDB	Q39WQ9.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	GMET269799:GMET_1075-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_GEOMG

Accession

Primary (citable) accession number: Q39WQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program