Reviewed, UniProtKB/Swiss-Prot Q39US3 (PYRC_GEOMG)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Gmet_1770

Organism

Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]

Taxonomic identifier

269799 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Dihydroorotase		PRO_0000325594
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1; via carbamate group By similarity
	Metal binding	143	1	Zinc 2; via carbamate group By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	233	1	Zinc 2 By similarity
	Metal binding	306	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	143	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39US3-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 0DC8AA0CE041462B	FASTA	424	44,713
	10 20 30 40 50 60 MNLLIQGGRV IDPSQGIDEV LDILVENGAV KELGKGVKAP SGTETIDASG LIVTPGLIDM 70 80 90 100 110 120 HVHLRDPGHE YKEDIVSGTK AAAAGGFTSV ACMPNTKPVN DNKAVTSYII AKAKAEGSVN 130 140 150 160 170 180 VFPVGSITQG SKGELLSEMG ELKESGCVAV SDDGHPVTNS ELMRRALEYA KGMGIMVISH 190 200 210 220 230 240 AEDLSLVGAG VMNEGFVSTE LGLKGIPWAA EDAATARDVY LAEFTDSPLH IAHVSTKGSL 250 260 270 280 290 300 RIIRNAKARG VKVTCETAPH YFSLTDDAVR GYDTNAKMNP PLRTADDVTA VKEALKDGTI 310 320 330 340 350 360 DAIATDHAPH HIDEKDLEFN EALNGIVGLE TSLTLSLRLV EEGVLTLPVL VDKMACNPAK 370 380 390 400 410 420 ILGIDRGTLK PGSVADITVI DPKATWTVDA DKLASKSKNS PFLGWEVKGA AAFTIVGGKV VYKR

References

[1]

"Complete sequence of Geobacter metallireducens GS-15." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000148 Genomic DNA. Translation: ABB32001.1. Different initiation.
RefSeq	YP_384726.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3741446.
GenomeReviews	Gene locus Gmet_1770 in contig CP000148_GR.
KEGG	gme:Gmet_1770.
NMPDR	fig|269799.3.peg.2087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39US3.
Enzyme and pathway databases
BioCyc	GMET269799:GMET_1770-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_GEOMG

Accession

Primary (citable) accession number: Q39US3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	September 2, 2008
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)