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Q39T99 (SYL_GEOMG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Gmet_2300
OrganismGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]
Taxonomic identifier269799 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009346

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q39T99 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 63EB85F98765DD2D

FASTA82493,514
        10         20         30         40         50         60 
MEEKYIPRNV EEKWQKIWEE NKTYKVTEDP SKPKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVGRFKRLRG FNVLHPMGWD AFGMPAENAA IQHKSHPAKW TYENIAYMRS QLKKMGLSYD 

       130        140        150        160        170        180 
WDRELATCDL DYYKWEQKVF LEMYEKGLAY KKTSYVNWCP KCETVLANEQ VEDGACWRCD 

       190        200        210        220        230        240 
SEVTQKELEQ WFFRITDYAE ELLEYTEKLP GWPERVLTMQ RNWIGKSYGC EIDFPVEGSL 

       250        260        270        280        290        300 
AKIKVFTTRQ DTLYGATFMS LAPEHPMALE LTTPDRRAEV EAFIDKVKKT DKIKRTAEDF 

       310        320        330        340        350        360 
EKEGVFTGSY CINPVTNRRM PVFLANFVLL DYGTGAVMAV PTHDQRDFEF ARKYDLPLQV 

       370        380        390        400        410        420 
VIQPEGETLD PAAMATAYTE VGTMVNSGTF DGLRSDEAKE KIADYLAKEG IGTKTVNFRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPVIYC DICGVVPVPE KDLPVVLPMD VEFTGEGGSP LKKLDSFVNV 

       490        500        510        520        530        540 
PCPQCGQMAR RETDTMDTFV QSSWYFLRYC CPDFAAGPID KARAEYWMSV DQYIGGIEHA 

       550        560        570        580        590        600 
VLHLLYARFF TKALRDLGYV TVDEPFTNLL TQGMVIKDGA KMSKSKGNVV DPDALINRYG 

       610        620        630        640        650        660 
ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLSRVWRLVC DLLPFVGKGG AVDSASLSDD 

       670        680        690        700        710        720 
ARGLRRAVHK TIRKVTDDID ERFHFNTAIA AIMELVNAIY AFEPKNAPEN GPVLTEAIES 

       730        740        750        760        770        780 
VVIMLSPFVP HVTEELWEAL GHQGGVEAAG WPSFDPSAAV DEEFLIVVQV NGKLRGKVTV 

       790        800        810        820 
ATDATEEQVK AAAFADEKVK PWIEGKQLRK AIYVPGKLLN IVVG 

« Hide

References

[1]"Complete sequence of Geobacter metallireducens GS-15."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GS-15 / ATCC 53774 / DSM 7210.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000148 Genomic DNA. Translation: ABB32525.1.
RefSeqYP_006721261.1. NC_007517.1.

3D structure databases

ProteinModelPortalQ39T99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269799.Gmet_2300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB32525; ABB32525; Gmet_2300.
GeneID3739328.
KEGGgme:Gmet_2300.
PATRIC22003856. VBIGeoMet55070_2341.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGMET269799:GHNY-2339-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEOMG
AccessionPrimary (citable) accession number: Q39T99
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries