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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGMET269799:GHNY-2339-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Gmet_2300
OrganismiGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Taxonomic identifieri269799 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000007073 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Leucine--tRNA ligasePRO_1000009346Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269799.Gmet_2300.

Structurei

3D structure databases

ProteinModelPortaliQ39T99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi581 – 5855"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39T99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKYIPRNV EEKWQKIWEE NKTYKVTEDP SKPKYYLLEM FPYPSGRIHM
60 70 80 90 100
GHVRNYSIGD VVGRFKRLRG FNVLHPMGWD AFGMPAENAA IQHKSHPAKW
110 120 130 140 150
TYENIAYMRS QLKKMGLSYD WDRELATCDL DYYKWEQKVF LEMYEKGLAY
160 170 180 190 200
KKTSYVNWCP KCETVLANEQ VEDGACWRCD SEVTQKELEQ WFFRITDYAE
210 220 230 240 250
ELLEYTEKLP GWPERVLTMQ RNWIGKSYGC EIDFPVEGSL AKIKVFTTRQ
260 270 280 290 300
DTLYGATFMS LAPEHPMALE LTTPDRRAEV EAFIDKVKKT DKIKRTAEDF
310 320 330 340 350
EKEGVFTGSY CINPVTNRRM PVFLANFVLL DYGTGAVMAV PTHDQRDFEF
360 370 380 390 400
ARKYDLPLQV VIQPEGETLD PAAMATAYTE VGTMVNSGTF DGLRSDEAKE
410 420 430 440 450
KIADYLAKEG IGTKTVNFRL RDWGISRQRY WGNPIPVIYC DICGVVPVPE
460 470 480 490 500
KDLPVVLPMD VEFTGEGGSP LKKLDSFVNV PCPQCGQMAR RETDTMDTFV
510 520 530 540 550
QSSWYFLRYC CPDFAAGPID KARAEYWMSV DQYIGGIEHA VLHLLYARFF
560 570 580 590 600
TKALRDLGYV TVDEPFTNLL TQGMVIKDGA KMSKSKGNVV DPDALINRYG
610 620 630 640 650
ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLSRVWRLVC DLLPFVGKGG
660 670 680 690 700
AVDSASLSDD ARGLRRAVHK TIRKVTDDID ERFHFNTAIA AIMELVNAIY
710 720 730 740 750
AFEPKNAPEN GPVLTEAIES VVIMLSPFVP HVTEELWEAL GHQGGVEAAG
760 770 780 790 800
WPSFDPSAAV DEEFLIVVQV NGKLRGKVTV ATDATEEQVK AAAFADEKVK
810 820
PWIEGKQLRK AIYVPGKLLN IVVG
Length:824
Mass (Da):93,514
Last modified:November 22, 2005 - v1
Checksum:i63EB85F98765DD2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB32525.1.
RefSeqiWP_004514498.1. NC_007517.1.
YP_006721261.1. NC_007517.1.

Genome annotation databases

EnsemblBacteriaiABB32525; ABB32525; Gmet_2300.
KEGGigme:Gmet_2300.
PATRICi22003856. VBIGeoMet55070_2341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB32525.1.
RefSeqiWP_004514498.1. NC_007517.1.
YP_006721261.1. NC_007517.1.

3D structure databases

ProteinModelPortaliQ39T99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269799.Gmet_2300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB32525; ABB32525; Gmet_2300.
KEGGigme:Gmet_2300.
PATRICi22003856. VBIGeoMet55070_2341.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciGMET269799:GHNY-2339-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Geobacter metallireducens GS-15."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS-15 / ATCC 53774 / DSM 7210.

Entry informationi

Entry nameiSYL_GEOMG
AccessioniPrimary (citable) accession number: Q39T99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.