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Protein

Phosphoribosylglycinamide formyltransferase 2

Gene

purT

Organism
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP.UniRule annotation

Catalytic activityi

Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoribosylglycinamide formyltransferase 2 (purT)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 8015'-phosphoribosylglycinamideUniRule annotation
Binding sitei112 – 1121ATPUniRule annotation
Binding sitei153 – 1531ATPUniRule annotation
Binding sitei201 – 2011ATPUniRule annotation
Metal bindingi265 – 2651MagnesiumUniRule annotation
Metal bindingi277 – 2771MagnesiumUniRule annotation
Binding sitei284 – 28415'-phosphoribosylglycinamideUniRule annotation
Binding sitei354 – 35415'-phosphoribosylglycinamideUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi158 – 1636ATPUniRule annotation
Nucleotide bindingi193 – 1964ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGMET269799:GHNY-3238-MONOMER.
UniPathwayiUPA00074; UER00127.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylglycinamide formyltransferase 2UniRule annotation (EC:2.1.2.-UniRule annotation)
Short name:
GART 2UniRule annotation
Alternative name(s):
5'-phosphoribosylglycinamide transformylase 2UniRule annotation
Formate-dependent GAR transformylaseUniRule annotation
GAR transformylase 2UniRule annotation
Gene namesi
Name:purTUniRule annotation
Ordered Locus Names:Gmet_3193
OrganismiGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Taxonomic identifieri269799 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000007073 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Phosphoribosylglycinamide formyltransferase 2PRO_0000319174Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi269799.Gmet_3193.

Structurei

3D structure databases

ProteinModelPortaliQ39QR8.
SMRiQ39QR8. Positions 1-389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini117 – 306190ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 2125'-phosphoribosylglycinamide bindingUniRule annotation
Regioni361 – 36225'-phosphoribosylglycinamide bindingUniRule annotation

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HH9. Bacteria.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.
OMAiIFGVEFF.
OrthoDBiPOG091H03TI.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01643. PurT. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005862. PurT.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01142. purT. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39QR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGTPLKKSA TRVMLLGSGE LGKEVVLEAQ RLGVEVIAVD RYADAPAMQV
60 70 80 90 100
AHRAHVVNML DRVELGRIVA RERPHLIVPE IEAIDTPYLL ELEQEGYTVI
110 120 130 140 150
PTARAANLTM NREGIRRLAA EELGLPTAAY RFAASIESFR AAVKDIGLPC
160 170 180 190 200
VVKPIMSSSG KGQSVVKSME EIDGAWTYAM EGGRGASDTV IVEEFIPFDY
210 220 230 240 250
EITLLTVRHA GGTTFCPPIG HVQIKGDYHE SWQPMAMTPA ALAESQRQAK
260 270 280 290 300
AVTDALGGSG IFGVELFIKG DRVWFSEVSP RPHDTGMVTM ISQNLSEFEL
310 320 330 340 350
HVRAILGLPV PEVANLAPAA SHVVLASEAA EEVTFSGLDA ALSVPETKLR
360 370 380 390
LFGKPDTRPG RRMGVALSFG ADTDEARNRA EQAAHAVKIV TL
Length:392
Mass (Da):42,283
Last modified:November 22, 2005 - v1
Checksum:i202F78BF2881AFB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB33406.1.
RefSeqiWP_004512631.1. NC_007517.1.

Genome annotation databases

EnsemblBacteriaiABB33406; ABB33406; Gmet_3193.
KEGGigme:Gmet_3193.
PATRICi22005644. VBIGeoMet55070_3228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB33406.1.
RefSeqiWP_004512631.1. NC_007517.1.

3D structure databases

ProteinModelPortaliQ39QR8.
SMRiQ39QR8. Positions 1-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269799.Gmet_3193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB33406; ABB33406; Gmet_3193.
KEGGigme:Gmet_3193.
PATRICi22005644. VBIGeoMet55070_3228.

Phylogenomic databases

eggNOGiENOG4108HH9. Bacteria.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.
OMAiIFGVEFF.
OrthoDBiPOG091H03TI.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00127.
BioCyciGMET269799:GHNY-3238-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01643. PurT. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005862. PurT.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01142. purT. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURT_GEOMG
AccessioniPrimary (citable) accession number: Q39QR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 22, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.