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Protein

Formate-dependent phosphoribosylglycinamide formyltransferase

Gene

purT

Organism
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.UniRule annotation

Catalytic activityi

Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Formate-dependent phosphoribosylglycinamide formyltransferase (purT)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei805'-phosphoribosylglycinamideUniRule annotation1
Binding sitei112ATPUniRule annotation1
Binding sitei153ATPUniRule annotation1
Binding sitei201ATPUniRule annotation1
Metal bindingi265MagnesiumUniRule annotation1
Metal bindingi277MagnesiumUniRule annotation1
Binding sitei2845'-phosphoribosylglycinamideUniRule annotation1
Binding sitei3545'-phosphoribosylglycinamideUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi158 – 163ATPUniRule annotation6
Nucleotide bindingi193 – 196ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00127.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate-dependent phosphoribosylglycinamide formyltransferaseUniRule annotation
Alternative name(s):
5'-phosphoribosylglycinamide transformylase 2UniRule annotation
Formate-dependent GAR transformylaseUniRule annotation (EC:2.1.2.-UniRule annotation)
GAR transformylase 2UniRule annotation
Short name:
GART 2UniRule annotation
Non-folate glycinamide ribonucleotide transformylaseUniRule annotation
Phosphoribosylglycinamide formyltransferase 2UniRule annotation
Gene namesi
Name:purTUniRule annotation
Ordered Locus Names:Gmet_3193
OrganismiGeobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Taxonomic identifieri269799 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000007073 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003191741 – 392Formate-dependent phosphoribosylglycinamide formyltransferaseAdd BLAST392

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi269799.Gmet_3193.

Structurei

3D structure databases

ProteinModelPortaliQ39QR8.
SMRiQ39QR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 306ATP-graspUniRule annotationAdd BLAST190

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 215'-phosphoribosylglycinamide bindingUniRule annotation2
Regioni361 – 3625'-phosphoribosylglycinamide bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HH9. Bacteria.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.
OMAiGMVTMIT.
OrthoDBiPOG091H03TI.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_01643. PurT. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR016185. PreATP-grasp_dom.
IPR005862. PurT.
PfamiView protein in Pfam
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01142. purT. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39QR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGTPLKKSA TRVMLLGSGE LGKEVVLEAQ RLGVEVIAVD RYADAPAMQV
60 70 80 90 100
AHRAHVVNML DRVELGRIVA RERPHLIVPE IEAIDTPYLL ELEQEGYTVI
110 120 130 140 150
PTARAANLTM NREGIRRLAA EELGLPTAAY RFAASIESFR AAVKDIGLPC
160 170 180 190 200
VVKPIMSSSG KGQSVVKSME EIDGAWTYAM EGGRGASDTV IVEEFIPFDY
210 220 230 240 250
EITLLTVRHA GGTTFCPPIG HVQIKGDYHE SWQPMAMTPA ALAESQRQAK
260 270 280 290 300
AVTDALGGSG IFGVELFIKG DRVWFSEVSP RPHDTGMVTM ISQNLSEFEL
310 320 330 340 350
HVRAILGLPV PEVANLAPAA SHVVLASEAA EEVTFSGLDA ALSVPETKLR
360 370 380 390
LFGKPDTRPG RRMGVALSFG ADTDEARNRA EQAAHAVKIV TL
Length:392
Mass (Da):42,283
Last modified:November 22, 2005 - v1
Checksum:i202F78BF2881AFB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000148 Genomic DNA. Translation: ABB33406.1.
RefSeqiWP_004512631.1. NC_007517.1.

Genome annotation databases

EnsemblBacteriaiABB33406; ABB33406; Gmet_3193.
KEGGigme:Gmet_3193.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPURT_GEOMG
AccessioniPrimary (citable) accession number: Q39QR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 22, 2005
Last modified: June 7, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families