Reviewed, UniProtKB/Swiss-Prot Q39QA7 (NUOA2_GEOMG)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 2
    NDH-1 subunit A 2
    NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	Gmet_3355

Organism

Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) [Complete proteome] [HAMAP]

Taxonomic identifier

269799 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity. HAMAP MF_01394
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000362692
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	87 – 107	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39QA7-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 6192FD0FAF14F785	FASTA	118	13,316
	10 20 30 40 50 60 MLGAYLPILV LVAIAVIFGL CSLVFSSLIG QKKPSVVKLA PYECGCEPVG SARERFSVKF 70 80 90 100 110 YIIAMLFILF DIEAVFLYPW SVLFKRLGMF GVMEMGVFIV ILFVGYIYVW KKGALEWE

References

[1]

"Complete sequence of Geobacter metallireducens GS-15." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Chain P., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000148 Genomic DNA. Translation: ABB33567.1.
RefSeq	YP_386292.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39QA7.
Genome annotation databases
GeneID	3739099.
GenomeReviews	Gene locus Gmet_3355 in contig CP000148_GR.
KEGG	gme:Gmet_3355.
NMPDR	fig|269799.3.peg.3330.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HBG605540.
OMA	CEPVGSA.
PhylomeDB	Q39QA7.
Enzyme and pathway databases
BioCyc	GMET269799:GMET_3355-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_GEOMG

Accession

Primary (citable) accession number: Q39QA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	November 22, 2005
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)