Reviewed, UniProtKB/Swiss-Prot Q39N89 (GLGB_BURS3)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Bcep18194_C7032

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000260639
Sites
	Active site	309	1	By similarity
	Active site	344	1	By similarity
	Active site	349	1	By similarity
	Active site	412	1	By similarity
	Active site	414	1	By similarity
	Active site	469	1	By similarity
	Active site	536	1	By similarity
	Active site	537	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39N89-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 4F416E1A2455363A	FASTA	735	80,677
	10 20 30 40 50 60 MTDTLFERSD IDALLAGRHP DPFACLGPHG QTGQVVVRAV LPGAKSVHAL SPEGAELGTL 70 80 90 100 110 120 ACVDRAGCFA GTIPRNGGAP HYLLAIDWPD ARQVIDDAYA FGTLLDDAVL ARFSAGDPAA 130 140 150 160 170 180 VLDCLGATPA RIDDTDGVRF AVWAPNAQRV SVVGDFNGWD GRRHPMRLRR PSGVWELFVP 190 200 210 220 230 240 GIGAGECYKY ELRAADGRVL PHKSDPCARA TEAPPRTASV VADVAALDAF AWHDEGWMHA 250 260 270 280 290 300 RPRADRYRVP WSIYEVHAES WQRVPEEMNR SATWDELAER LIPYVQGMGF THVEFMPIAE 310 320 330 340 350 360 YPFGGSWGYQ PVAQFAPSAR FGPVDGFARF VDRAHAAGIG VLVDWVPAHF PDDPHGLAQF 370 380 390 400 410 420 DGTALYEHAD PREGLHPDWH TCVFNVGRTE VGAFLAASAL AWARRYHVDG IRVDAVASML 430 440 450 460 470 480 YRDYSRAEGE WVPNVHGGRE NLESVAFLRM LNDTLHGAAA PAGVVTVAEE STAWPGVTAP 490 500 510 520 530 540 TGDGGLGFDF KWNMGWMHDT LAYVREDPVH RRYHHDRMTF GLVYAFSERF VLPLSHDEVV 550 560 570 580 590 600 HGKGSLVAKM PGDAWQRLAT LRAYFGFMWA HPGKKLLFMG SEFAQWAEFA HDATPHWDLL 610 620 630 640 650 660 DAPAHRGVQR LVRDLNRTYA AEPALHALDC HAAGFSWLIG DDRDNSVFAF ARRDEAGHLV 670 680 690 700 710 720 VAICNFTPVP RASYRVGLPA PGQWRELMNT DAAPYGGTNA GNDGAVWAEA VPAHGEAWSA 730 LLRLPPLATL WLRPA

References

[1]

"Complete sequence of chromosome 3 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000150 Genomic DNA. Translation: ABB06077.1.
RefSeq	YP_366721.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39N89.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3734484.
GenomeReviews	Gene locus Bcep18194_C7032 in contig CP000150_GR.
KEGG	bur:Bcep18194_C7032.
NMPDR	fig|269483.3.peg.3399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39N89.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_C7032-MON.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURS3

Accession

Primary (citable) accession number: Q39N89

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)