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Q39N89 (GLGB_BURS3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Bcep18194_C7032
OrganismBurkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]
Taxonomic identifier482957 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7357351,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260639

Sites

Active site4141Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q39N89 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 4F416E1A2455363A

FASTA73580,677
        10         20         30         40         50         60 
MTDTLFERSD IDALLAGRHP DPFACLGPHG QTGQVVVRAV LPGAKSVHAL SPEGAELGTL 

        70         80         90        100        110        120 
ACVDRAGCFA GTIPRNGGAP HYLLAIDWPD ARQVIDDAYA FGTLLDDAVL ARFSAGDPAA 

       130        140        150        160        170        180 
VLDCLGATPA RIDDTDGVRF AVWAPNAQRV SVVGDFNGWD GRRHPMRLRR PSGVWELFVP 

       190        200        210        220        230        240 
GIGAGECYKY ELRAADGRVL PHKSDPCARA TEAPPRTASV VADVAALDAF AWHDEGWMHA 

       250        260        270        280        290        300 
RPRADRYRVP WSIYEVHAES WQRVPEEMNR SATWDELAER LIPYVQGMGF THVEFMPIAE 

       310        320        330        340        350        360 
YPFGGSWGYQ PVAQFAPSAR FGPVDGFARF VDRAHAAGIG VLVDWVPAHF PDDPHGLAQF 

       370        380        390        400        410        420 
DGTALYEHAD PREGLHPDWH TCVFNVGRTE VGAFLAASAL AWARRYHVDG IRVDAVASML 

       430        440        450        460        470        480 
YRDYSRAEGE WVPNVHGGRE NLESVAFLRM LNDTLHGAAA PAGVVTVAEE STAWPGVTAP 

       490        500        510        520        530        540 
TGDGGLGFDF KWNMGWMHDT LAYVREDPVH RRYHHDRMTF GLVYAFSERF VLPLSHDEVV 

       550        560        570        580        590        600 
HGKGSLVAKM PGDAWQRLAT LRAYFGFMWA HPGKKLLFMG SEFAQWAEFA HDATPHWDLL 

       610        620        630        640        650        660 
DAPAHRGVQR LVRDLNRTYA AEPALHALDC HAAGFSWLIG DDRDNSVFAF ARRDEAGHLV 

       670        680        690        700        710        720 
VAICNFTPVP RASYRVGLPA PGQWRELMNT DAAPYGGTNA GNDGAVWAEA VPAHGEAWSA 

       730 
LLRLPPLATL WLRPA 

« Hide

References

[1]"Complete sequence of chromosome 3 of Burkholderia sp. 383."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 383.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000150 Genomic DNA. Translation: ABB06077.1.
RefSeqYP_366721.1. NC_007509.1.

3D structure databases

ProteinModelPortalQ39N89.
SMRQ39N89. Positions 124-734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269483.Bcep18194_C7032.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB06077; ABB06077; Bcep18194_C7032.
GeneID3734484.
KEGGbur:Bcep18194_C7032.
PATRIC19282118. VBIBurSp120713_0518.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBSP269483:GHLS-7116-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BURS3
AccessionPrimary (citable) accession number: Q39N89
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 22, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries