Reviewed, UniProtKB/Swiss-Prot Q39LI1 (3HAO_BURS3)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

Bcep18194_C7641

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde.
Cofactor	Binds 2 Fe(2+) ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	3-hydroxyanthranilate 3,4-dioxygenase		PRO_0000245474
Sites
	Metal binding	51	1	Iron 1; catalytic By similarity
	Metal binding	57	1	Iron 1; catalytic By similarity
	Metal binding	95	1	Iron 1; catalytic By similarity
	Metal binding	125	1	Iron 2 By similarity
	Metal binding	128	1	Iron 2 By similarity
	Metal binding	162	1	Iron 2 By similarity
	Metal binding	165	1	Iron 2 By similarity
	Binding site	47	1	Dioxygen By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39LI1-1 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: FB19D9C29561A99B	FASTA	174	20,048
	10 20 30 40 50 60 MLKYGTPFNF SRWIDEHAHL LKPPVGNQQV WQDSDFIVTV VGGPNHRTDY HDDPFEEFFY 70 80 90 100 110 120 QLRGNAYLHL WIDGKRERVD LKEGDMFLLP PHVRHSPQRP EAGSACLVIE RQRPAGVVDG 130 140 150 160 170 FEWYCDACGH LVHRVEVQLK SIVDDLPPLF DAFYASDTLR RCAHCGHMHP GKAT

References

[1]

"Complete sequence of chromosome 3 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000150 Genomic DNA. Translation: ABB06685.1. Different initiation.
RefSeq	YP_367329.1.
3D structure databases
SMR	Q39LI1. Positions 1-173.
ModBase	Search...
Genome annotation databases
GeneID	3734508.
GenomeReviews	Gene locus Bcep18194_C7641 in contig CP000150_GR.
KEGG	bur:Bcep18194_C7641.
NMPDR	fig|269483.3.peg.4346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39LI1.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_C7641-MON.
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_BURS3

Accession

Primary (citable) accession number: Q39LI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	November 4, 2008
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)