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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase 2

Gene

panB2

Organism
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase 2 (panB2), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase 1 (panB1)
  2. 2-dehydropantoate 2-reductase (WJ23_32535), 2-dehydropantoate 2-reductase (WJ23_19030), 2-dehydropantoate 2-reductase (WJ23_13905), 2-dehydropantoate 2-reductase (VL00_11020), 2-dehydropantoate 2-reductase (VL00_32420), 2-dehydropantoate 2-reductase (Bcep18194_A5912), 2-dehydropantoate 2-reductase (Bcep18194_A5870), 2-dehydropantoate 2-reductase (VL00_07945), 2-dehydropantoate 2-reductase (WJ23_13680), 2-dehydropantoate 2-reductase (Bcep18194_B0882)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53MagnesiumUniRule annotation1
Metal bindingi92MagnesiumUniRule annotation1
Binding sitei92Alpha-ketoisovalerateUniRule annotation1
Binding sitei120Alpha-ketoisovalerateUniRule annotation1
Metal bindingi122MagnesiumUniRule annotation1
Active sitei189Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSP269483:GHLS-683-MONOMER.
UniPathwayiUPA00028; UER00003.

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 2UniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferase 2UniRule annotation
Short name:
KPHMT 2UniRule annotation
Gene namesi
Name:panB2UniRule annotation
Ordered Locus Names:Bcep18194_A3842
OrganismiBurkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002972361 – 2713-methyl-2-oxobutanoate hydroxymethyltransferase 2Add BLAST271

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ39JC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078427.
KOiK00606.
OMAiQMAYHTE.
OrthoDBiPOG091H02K8.

Family and domain databases

CDDicd06557. KPHMT-like. 1 hit.
Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB. 1 hit.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39JC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYLQESSRP AVTVPKLQAM RDAGEKIAML TCYDASFSAL LDRSGVDVLL
60 70 80 90 100
IGDSLGNVLQ GHTTTLPVSL DDIAYHTACV ARAQPRALIM ADLPFGTYGT
110 120 130 140 150
PAEAFASSVK LMRAGAQMVK LEGGEWLAET IRFLVERAVP VCAHVGLTPQ
160 170 180 190 200
SVHAFGGFKV QGKTEAGAAQ LLRDARAVEA AGAQVVLLEA VPTLIGSEVT
210 220 230 240 250
HMLRVPTIGI GAGADCSGQV LVLHDMLGVF PGKRPRFVKD FMQGEPNIQA
260 270
AVEAYVRAVK DGSFPGPEHS F
Length:271
Mass (Da):28,686
Last modified:November 22, 2005 - v1
Checksum:i07EF436360F36A6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB07441.1.
RefSeqiWP_011351027.1. NC_007510.1.

Genome annotation databases

EnsemblBacteriaiABB07441; ABB07441; Bcep18194_A3842.
KEGGibur:Bcep18194_A3842.
PATRICi19285008. VBIBurSp120713_1936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB07441.1.
RefSeqiWP_011351027.1. NC_007510.1.

3D structure databases

ProteinModelPortaliQ39JC5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB07441; ABB07441; Bcep18194_A3842.
KEGGibur:Bcep18194_A3842.
PATRICi19285008. VBIBurSp120713_1936.

Phylogenomic databases

HOGENOMiHOG000078427.
KOiK00606.
OMAiQMAYHTE.
OrthoDBiPOG091H02K8.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003.
BioCyciBSP269483:GHLS-683-MONOMER.

Family and domain databases

CDDicd06557. KPHMT-like. 1 hit.
Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB. 1 hit.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPANB2_BURL3
AccessioniPrimary (citable) accession number: Q39JC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: November 22, 2005
Last modified: October 5, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.