Q39JC5 (PANB2_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 2
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase 2
Short name=KPHMT 2

Gene names

Name:	panB2
Ordered Locus Names:	Bcep18194_A3842

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	3-methyl-2-oxobutanoate hydroxymethyltransferase 2 HAMAP MF_00156		PRO_0000297236
Regions
	Region	53 – 54	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Metal binding	53	1	Magnesium By similarity
	Metal binding	92	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	92	1	Alpha-ketoisovalerate By similarity
	Binding site	120	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39JC5 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 07EF436360F36A6C	FASTA	271	28,686
	10 20 30 40 50 60 MTYLQESSRP AVTVPKLQAM RDAGEKIAML TCYDASFSAL LDRSGVDVLL IGDSLGNVLQ 70 80 90 100 110 120 GHTTTLPVSL DDIAYHTACV ARAQPRALIM ADLPFGTYGT PAEAFASSVK LMRAGAQMVK 130 140 150 160 170 180 LEGGEWLAET IRFLVERAVP VCAHVGLTPQ SVHAFGGFKV QGKTEAGAAQ LLRDARAVEA 190 200 210 220 230 240 AGAQVVLLEA VPTLIGSEVT HMLRVPTIGI GAGADCSGQV LVLHDMLGVF PGKRPRFVKD 250 260 270 FMQGEPNIQA AVEAYVRAVK DGSFPGPEHS F

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB07441.1.
RefSeq	YP_368085.1. NC_007510.1.
3D structure databases
ProteinModelPortal	Q39JC5.
SMR	Q39JC5. Positions 12-271.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39JC5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3749026.
GenomeReviews	Gene locus Bcep18194_A3842 in contig CP000151_GR.
KEGG	bur:Bcep18194_A3842.
NMPDR	fig|269483.3.peg.4559.
PATRIC	19285008. VBIBurSp120713_1936.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	YDATFAH.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A3842-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB2_BURS3

Accession

Primary (citable) accession number: Q39JC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program