Reviewed, UniProtKB/Swiss-Prot Q39J72 (GLYA3_BURS3)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 3
      Short name=Serine methylase 3
      Short name=SHMT 3
    EC=2.1.2.1

Gene names

Name:	glyA3
Ordered Locus Names:	Bcep18194_A3895

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Serine hydroxymethyltransferase 3 HAMAP MF_00051		PRO_0000234959
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39J72-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 949B4C6534C0A5B9	FASTA	415	44,842
	10 20 30 40 50 60 MFDRAQSTIA NVDPEIFAAI EQENRRQEDH IELIASENYT SPAVMAAQGS QLTNKYAEGY 70 80 90 100 110 120 PGKRYYGGCE YVDVVEQLAI DRVKQLFGAE AANVQPNSGS QANQGVFFAM LKPGDTIMGM 130 140 150 160 170 180 SLAHGGHLTH GSPVNMSGKW FNVVSYGLNE NEDIDYEAAE QLAQEHKPKL IVAGASAFSL 190 200 210 220 230 240 KIDFERLAKI AKSVGAYLMV DMAHYAGLIA AGVYPNPVPH ADFVTTTTHK SLRGPRGGVI 250 260 270 280 290 300 LMKAEYEKPI NSAIFPGIQG GPLMHVIAGK AVAFKEALSP EFKAYQEKVV ENARVLAETL 310 320 330 340 350 360 VKRGLRIVSG RTESHVMLVD LRAKHITGKA AEAALGAAHI TVNKNAIPND PEKPFVTSGV 370 380 390 400 410 RLGSPAMTTR GFGPAEAEQV GNLIADVLDN PEDAATIERV RAQVAELTKR FPVYR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB07494.1.
RefSeq	YP_368138.1.
3D structure databases
SMR	Q39J72. Positions 4-414.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39J72.
Genome annotation databases
GeneID	3749080.
GenomeReviews	Gene locus Bcep18194_A3895 in contig CP000151_GR.
KEGG	bur:Bcep18194_A3895.
NMPDR	fig|269483.3.peg.4639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0112.
HOGENOM	HBG301263.
OMA	FALRIDF.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A3895-MONOMER.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA3_BURS3

Accession

Primary (citable) accession number: Q39J72

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 22, 2005
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)