Reviewed, UniProtKB/Swiss-Prot Q39IE1 (DADA_BURS3)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Bcep18194_A4178

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066083
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39IE1-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: D4C011F2203689C3	FASTA	428	45,978
	10 20 30 40 50 60 MRVVILGSGV VGVASAYYLA RAGHEVTVID REAGPALETS FANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAVKWMFE KHAPLAIRLD GTRFQLQWMY QMLRNCTAER YAVNKGRMVR LAEYSRDCLQ 130 140 150 160 170 180 ALRADTGIQY EGRTGGTLQL FRTQQQLDGA AKDIAVLQEA NVPFELLSPA DLKKAEPALA 190 200 210 220 230 240 AVSHKLTGGL RLPGDETGDC QLFTTRLAAL AESLGVKFRY NTPIDALAIA GGKIAGVQCG 250 260 270 280 290 300 SETVRADAYV VALGSYSTSF ISNLMKIPVY PLKGYSITAP IVNEAAAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDQRIRV GGMAEIVGFD KKLRAARRET LEMCVNDLFP GGGDTSKATF WTGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPV SNLFLNTGHG TLGWTMSCGS GQLLADLISG KMPAIQADDL SVHRYLKDVA GQTRPAYA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000151 Genomic DNA. Translation: ABB07775.1.
RefSeq	YP_368419.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39IE1.
Genome annotation databases
GeneID	3749371.
GenomeReviews	Gene locus Bcep18194_A4178 in contig CP000151_GR.
KEGG	bur:Bcep18194_A4178.
NMPDR	fig|269483.3.peg.5348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39IE1.
OMA	MFQKHAP.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A4178-MON.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_BURS3

Accession

Primary (citable) accession number: Q39IE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)