Reviewed, UniProtKB/Swiss-Prot Q39H51 (SYFA_BURS3)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Bcep18194_A4619

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000231970
Sites
	Metal binding	266	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39H51-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 49567DDC9784743F	FASTA	345	38,958
	10 20 30 40 50 60 MTLEMMGSMD LDQIVADAQQ SFEQAADITT LENEKARFLG KSGALTELLK GLGKLDPEAR 70 80 90 100 110 120 KTEGARINVV KQQVEAALTA RRQALADALL NQRLTAEAID VTLPGRGAGA GSLHPVMRTW 130 140 150 160 170 180 ERVEQIFGSI GFDVADGPEI ETDWYNFTSL NSPENHPARS MQDTFYVEGK DADGRQLLLR 190 200 210 220 230 240 THTSPMQVRY ARMNRPPIKV IAPGRTYRVD SDATHSPMFN QVEGLWIDEN ISFADLKGVY 250 260 270 280 290 300 TDFLKKFFER DDILVRFRPS YFPFTEPSAE IDMMFEQGKN AGKWLEISGS GQVHPTVIRN 310 320 330 340 MGLDPERYIG FAFGSGLERL TMLRYGVQDL RLFFENDLRF LRQFA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000151 Genomic DNA. Translation: ABB08215.1.
RefSeq	YP_368859.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3749821.
GenomeReviews	Gene locus Bcep18194_A4619 in contig CP000151_GR.
KEGG	bur:Bcep18194_A4619.
NMPDR	fig|269483.3.peg.5939.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39H51.
OMA	Q39H51. FRASYFP.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A4619-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR002319. Phe-tRNA-synth_IIc-rel. IPR004529. Phe-tRNA-synth_IIc_asu. IPR018157. Phe-tRNA-synth_IIc_C. IPR004188. Phe-tRNA_synth_II_N. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BURS3

Accession

Primary (citable) accession number: Q39H51

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)