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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:Bcep18194_A4643
OrganismiBurkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002293461 – 322tRNA pseudouridine synthase BAdd BLAST322

Structurei

3D structure databases

ProteinModelPortaliQ39H28.
SMRiQ39H28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiPOG091H02G9.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiView protein in InterPro
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiView protein in Pfam
PF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39H28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPRTTELD RPMTTAASQR PRVPRRLLDG VLLLDKPVGL SSNDALIRAK
60 70 80 90 100
RLLLAKKAGH TGTLDPLASG LLPLCFGEAT KFSQDLLEAD KTYEATMRLG
110 120 130 140 150
QRTATGDAEG EVIDTRPVEC DRAAVEAALV RFTGEIVQVP PMYSALKRDG
160 170 180 190 200
KPLYEYARAG QTVEREGRNV TILALALLAC DLPDVTFRVT CSKGTYVRTL
210 220 230 240 250
AEDIGEALGC GAHLTMLRRT GVGALTLEHA VTLDALSDAD DASRDAWLQP
260 270 280 290 300
VDALLSTFPL VRLDETSAKR FLHGQRLPLS ALDPIDAAEG ERVRVYDATR
310 320
LLGVARKANG VLAPERLVVT AA
Length:322
Mass (Da):34,803
Last modified:November 22, 2005 - v1
Checksum:i841C8351C88B5F6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB08238.1.

Genome annotation databases

EnsemblBacteriaiABB08238; ABB08238; Bcep18194_A4643.
KEGGibur:Bcep18194_A4643.
PATRICi19286686. VBIBurSp120713_2756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB08238.1.

3D structure databases

ProteinModelPortaliQ39H28.
SMRiQ39H28.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB08238; ABB08238; Bcep18194_A4643.
KEGGibur:Bcep18194_A4643.
PATRICi19286686. VBIBurSp120713_2756.

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiPOG091H02G9.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiView protein in InterPro
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiView protein in Pfam
PF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_BURL3
AccessioniPrimary (citable) accession number: Q39H28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 22, 2005
Last modified: March 15, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.