Reviewed, UniProtKB/Swiss-Prot Q39FS0 (PURA1_BURS3)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase 1
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 1
    AdSS 1
    AMPSase 1

Gene names

Name:	purA1
Ordered Locus Names:	Bcep18194_A5102

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Adenylosuccinate synthetase 1 HAMAP MF_00011		PRO_0000224264
Regions
	Nucleotide binding	22 – 28	7	GTP Potential
Sites
	Active site	150	1	By similarity
	Active site	157	1	By similarity
	Metal binding	23	1	Magnesium By similarity
	Metal binding	50	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39FS0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 59ABC05C2C92CDBC	FASTA	448	48,133
	10 20 30 40 50 60 MSASAVNVTP GRNVVVVGTQ WGDEGKGKIV DWLTDHAQGV VRFQGGHNAG HTLIIGGKKT 70 80 90 100 110 120 ILRLIPSGIM REGVACYIGN GVVLSPEALF KEIGELEEAG VNVRDRLFIS EATTLILPYH 130 140 150 160 170 180 IAIDQAREAR KGAGKIGTTG RGIGPAYEDK VGRRALRVQD LFDAKTFADR LRENLDFHNF 190 200 210 220 230 240 VLTQYLGGAA VDFQATLDTM LGYADRLKPM VADVSRRLYD ANNAGQNLLF EGAQGTLLDI 250 260 270 280 290 300 DHGTYPFVTS SNCVAGAASA GAGVGPQKLN YILGITKAYC TRVGSGPFPS ELYDADNPQR 310 320 330 340 350 360 QDQVGVTLAN VGKEFGSVTG RPRRTGWLDA AALRRSIQIN GVSGLCMTKL DVLDGLDEVK 370 380 390 400 410 420 LCVGYKIDGK DADILPRGAA DVARCEPVYE TFAGWKESTV GIKTWEALPA NAQAYLTRVQ 430 440 EVAGVPVDMV STGPDRDETI LLRHPFKV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB08696.1.
RefSeq	YP_369340.1.
3D structure databases
SMR	Q39FS0. Positions 11-447.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39FS0.
Genome annotation databases
GeneID	3750310.
GenomeReviews	Gene locus Bcep18194_A5102 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5102.
NMPDR	fig|269483.3.peg.6177.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	YVLGIIK.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5102-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA1_BURS3

Accession

Primary (citable) accession number: Q39FS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)