Q39F53 (LPXD_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Bcep18194_A5319

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264352
Sites
	Active site	253	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39F53 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: C43C9A2AB00F099F	FASTA	359	36,582
	10 20 30 40 50 60 MALTLEELVK RFGGEIAGDA QCKVGGLAPL DQAGPQQLAF LANPKYLSQV ESTRAGAVLI 70 80 90 100 110 120 APKDLEKLGA AAGGRNFIVT PNPYAYFARV AQMFIDLATP QRAAGVHPSA TIDPAAQVAA 130 140 150 160 170 180 SAVIGPHVTV EAGAVIEDGV QLDANVFVGR GTTIGAGSHL YPNASVYHGC KVGPRAIIHA 190 200 210 220 230 240 GAVIGSDGFG FAPDFVGDGD ARTGSWVKIP QVGGVTIGPD VEIGANTTID RGAMADTVIE 250 260 270 280 290 300 ECVKIDNQVQ IGHNCRIGAY TVIAGSAGIA GSTTIGRHCM IGGAAGIAGH VTLGDYVIIT 310 320 330 340 350 AKSGVSKSLP KAGIYTSAFP AVDHGEWNKS AALVRNLDKL RERIKALEAA LAAQGGTDA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB08913.1.
RefSeq	YP_369557.1. NC_007510.1.
3D structure databases
ProteinModelPortal	Q39F53.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39F53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3750530.
GenomeReviews	Gene locus Bcep18194_A5319 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5319.
NMPDR	fig|269483.3.peg.6656.
PATRIC	19288042. VBIBurSp120713_3421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5319-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 6 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BURS3

Accession

Primary (citable) accession number: Q39F53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program