Reviewed, UniProtKB/Swiss-Prot Q39EY8 (SYC2_BURS3)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	Bcep18194_A5384

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000240897
Regions
	Motif	32 – 42	11	"HIGH" region HAMAP MF_00041
	Motif	271 – 275	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	30	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39EY8-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: F06E6443F732E9B1	FASTA	465	52,209
	10 20 30 40 50 60 MESLRIYNTL ARDKQVFVPR QSGEVRMYVC GITVYDYCHV GHARMLVVFD LVQRWLRAIG 70 80 90 100 110 120 YRVTYVRNIT DIDDKIIRRA VENGETIKSL TDRFIGAMHD DESALGIQRP DIEPRATEFI 130 140 150 160 170 180 PQMLGMIETL ETNGYAYQAT DGDVNYSVRK FANYGKLSGK SLDDLRAGER VAANDAKEDP 190 200 210 220 230 240 LDFVLWKRAK PEDPEGTSWA SKYGMGRPGW HIECSAMGCT LLGEHFDIHG GGQDLQFPHH 250 260 270 280 290 300 ENEIAQSEGA TGQTFVNYWM HNGFVQVDNE KMSKSLGNFF TIREVLERYD AEVMRFFIVR 310 320 330 340 350 360 THYRSPLNYS DVHLDDARAS LTRLYTALKD VEADTLALDW NEPHAQRFAA AMNDDFNTPV 370 380 390 400 410 420 AVATLFELAG EVNRTRDASL ARQLKQLAGL LGLLGREPRA FLQQASGAAQ AGGLAADEIE 430 440 450 460 AKIAARVAAK QAKDYAEADR IRAELLETGI ALEDKPGGST EWRRV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000151 Genomic DNA. Translation: ABB08978.1.
RefSeq	YP_369622.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39EY8.
Genome annotation databases
GeneID	3750603.
GenomeReviews	Gene locus Bcep18194_A5384 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5384.
NMPDR	fig|269483.3.peg.6761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39EY8.
OMA	VLWKAAK.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5384-MON.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_BURS3

Accession

Primary (citable) accession number: Q39EY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)