Reviewed, UniProtKB/Swiss-Prot Q39DS9 (ILVD2_BURS3)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	Bcep18194_A5793

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000225380
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39DS9-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: A2430DA07FCCD63F	FASTA	557	59,243
	10 20 30 40 50 60 MAYNRRSKHI TQGVARSPNR SMYYALGYQK DDFDKPMVGI ANGHSTITPC NSGLQRLSDA 70 80 90 100 110 120 AVAAVKAADA NPQIFGTPTI SDGMSMGTEG MKYSLVSREV IADCIETCVQ GQWMDGVVVV 130 140 150 160 170 180 GGCDKNMPGG MIALARLNVP GIYVYGGTIR PGNWKGRDLT IVSSFEAVGE FTAGRMSQED 190 200 210 220 230 240 FEGVERNACP TSGSCGGMYT ANTMSSSFEA LGMSLLYSST MANPDQEKVD SAAESARVLV 250 260 270 280 290 300 EAVKRDLKPR DIITKESIEN AVSVIMATGG STNAVLHYLA IAHAAEVDWT IEDFERIRKR 310 320 330 340 350 360 VPVICDLKPS GKYVATDLHR AGGIPQVLKI LLDAGLLHGD CMTITGRTIA DELKDVPSVP 370 380 390 400 410 420 RADQDVIFPI DRALYKEGHL AILKGNLAED GAVAKITGLK NPVITGPARV FDDEQSAMDA 430 440 450 460 470 480 ILGDRIRAGD ILVLRYLGPK GGPGMPEMLA PTSAIIGKGL GESVGFITDG RFSGGTWGMV 490 500 510 520 530 540 VGHVAPEAFV GGTIALVQEG DSITIDAHRL LLQLNVDDAE LARRRAAWQQ PAPRYTRGVL 550 AKFAALARPA NQGAVTG

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB09387.1.
RefSeq	YP_370031.1.
3D structure databases
SMR	Q39DS9. Positions 66-556.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39DS9.
Genome annotation databases
GeneID	3751023.
GenomeReviews	Gene locus Bcep18194_A5793 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5793.
NMPDR	fig|269483.3.peg.6977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NMPGAMI.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5793-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_BURS3

Accession

Primary (citable) accession number: Q39DS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)