Reviewed, UniProtKB/Swiss-Prot Q39DS5 (AMPA_BURS3)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bcep18194_A5797

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019899
Sites
	Active site	286	1	Potential
	Active site	360	1	Potential
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 1 By similarity
	Metal binding	279	1	Manganese 2 By similarity
	Metal binding	297	1	Manganese 2 By similarity
	Metal binding	356	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39DS5-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: D54597BE428DA025	FASTA	503	53,037
	10 20 30 40 50 60 MDFSIKGCDW SKGEAKGFLT GKSDCIVLGI FEAQTLSGAA LDIDTATKGL ISRVIKAGDM 70 80 90 100 110 120 DGKRGKTLFL HEVSGIGASR VLLVGLGKQD AFNQKAYNDA VTAAWRALLA TKVVQVTFTL 130 140 150 160 170 180 AQLPVDERSS DWGVRAAILA LRNETYRFTQ MKSKPEPASH TLKRVVFSVD PSDEKAAKVA 190 200 210 220 230 240 VKQAVALANG MDLTRDLGNL PGNVCTPTYL GNTAKKIAKD WGLKAEVLGL KQIQALKMGS 250 260 270 280 290 300 FLSVARASVE PPQFIVLHYQ GAAAKAAPVV LVGKGITFDT GGISLKPGEG MDEMKYDMCG 310 320 330 340 350 360 AGSVLGTMRA VAEMGLKINV VAIVPTCENM PGGNATKPGD IVTSMKGLTI EVLNTDAEGR 370 380 390 400 410 420 LILCDALTYA ERFKPAAVID VATLTGACVI ALGGHNSGLF STDDALAGEL LDASREANDP 430 440 450 460 470 480 AWRMPLDDEY QDQLKSNFAD LANIGGRPAG AVTAACFLSR FTESYPWAHL DIAGTAWKGG 490 500 AAKGATGRPV PLLAQFLIDR AGQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB09391.1.
RefSeq	YP_370035.1.
3D structure databases
HSSP	HSSP built from PDB template 2EWB based on UniProtKB P00727.
SMR	Q39DS5. Positions 20-502.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39DS5.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	3751027.
GenomeReviews	Gene locus Bcep18194_A5797 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5797.
NMPDR	fig|269483.3.peg.7033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	LGHHNSG.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5797-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BURS3

Accession

Primary (citable) accession number: Q39DS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)