Reviewed, UniProtKB/Swiss-Prot Q39DM8 (SYI_BURS3)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	Bcep18194_A5844

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	945 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 945	945	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_1000022048
Regions
	Motif	66 – 76	11	"HIGH" region HAMAP MF_02002
	Motif	622 – 626	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	908	1	Zinc By similarity
	Metal binding	911	1	Zinc By similarity
	Metal binding	928	1	Zinc By similarity
	Metal binding	931	1	Zinc By similarity
	Binding site	581	1	Aminoacyl-adenylate By similarity
	Binding site	625	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39DM8-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: C6F393E268E23A29	FASTA	945	105,866
	10 20 30 40 50 60 MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGIYEKIR AASAGRPKFI 70 80 90 100 110 120 LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV PGWDCHGMPI EIQIEKQFGK 130 140 150 160 170 180 SLPAAEVMSK ARAYATEQIE KQKVGFKRLG VLGDWANPYK TMNFVNEAEE LRALGKIIEK 190 200 210 220 230 240 GYVYRGLKPV NWCFDCGSAL AEAEVEYKDR TDPTIDVMFA FAEPEKTAQA FGLPALPRAE 250 260 270 280 290 300 GGIVIWTTTP WTIPANQALN LHPEIVYALV DTERGLLIIA EERVEACMTD FKLTGRVVAT 310 320 330 340 350 360 APGVKLAGLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG IEDFVSCKAH 370 380 390 400 410 420 GMTDSDFINP VMGDGRYIES LPLFGGLSIW DANPKIVEAL NAAGSLLRSE KYTHSYMHCW 430 440 450 460 470 480 RHKTPIIYRA TSQWFAGMDV TPRDGGKTLR ETALEGVDAT AFYPSWGKQR LFSMIANRPD 490 500 510 520 530 540 WTLSRQRQWG VPMAFFVHKE TGELHPRTLE LLEEVAKRVE QSGIEAWQTL DPRELIGDDA 550 560 570 580 590 600 NLYEKNRDTL DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASMI 610 620 630 640 650 660 DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA STDYSGELAI 670 680 690 700 710 720 SEEILKRVTE GYRRIRNTLR FLLANLSDFD FAQHAVPVDE WLEIDRYAVA FSQQLQTELL 730 740 750 760 770 780 GHYEKYEFHP VVAKLQTYCS EDLGGFYLDV LKDRLYTSAA DSRARRSAQT ALYHLTHGLL 790 800 810 820 830 840 RVLAPFLSFT AEEAWKVFQP ASDTIYTETY YAYPEVAGSA ALIEKWALLR DVRGNVTKAL 850 860 870 880 890 900 EEARTANRIG SSLQAEVAVH ASGARYDALT SLGDDLKFVL ITSAATVVKV DDEAQESVDV 910 920 930 940 AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE IRSAA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB09438.1.
RefSeq	YP_370082.1.
3D structure databases
SMR	Q39DM8. Positions 13-935.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39DM8.
Genome annotation databases
GeneID	3751075.
GenomeReviews	Gene locus Bcep18194_A5844 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5844.
NMPDR	fig|269483.3.peg.6621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HBG577712.
OMA	KQVLTHG.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5844-MONOMER.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BURS3

Accession

Primary (citable) accession number: Q39DM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)