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Q39DM8

- SYI_BURS3

UniProt

Q39DM8 - SYI_BURS3

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Bcep18194_A5844
Organism
Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylate By similarity
Binding sitei625 – 6251ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSP269483:GHLS-2731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Bcep18194_A5844
OrganismiBurkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194))
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002705: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022048Add
BLAST

Proteomic databases

PRIDEiQ39DM8.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi269483.Bcep18194_A5844.

Structurei

3D structure databases

ProteinModelPortaliQ39DM8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotation
Add
BLAST
Motifi622 – 6265"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39DM8-1 [UniParc]FASTAAdd to Basket

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MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGIYEKIR    50
AASAGRPKFI LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV 100
PGWDCHGMPI EIQIEKQFGK SLPAAEVMSK ARAYATEQIE KQKVGFKRLG 150
VLGDWANPYK TMNFVNEAEE LRALGKIIEK GYVYRGLKPV NWCFDCGSAL 200
AEAEVEYKDR TDPTIDVMFA FAEPEKTAQA FGLPALPRAE GGIVIWTTTP 250
WTIPANQALN LHPEIVYALV DTERGLLIIA EERVEACMTD FKLTGRVVAT 300
APGVKLAGLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG 350
IEDFVSCKAH GMTDSDFINP VMGDGRYIES LPLFGGLSIW DANPKIVEAL 400
NAAGSLLRSE KYTHSYMHCW RHKTPIIYRA TSQWFAGMDV TPRDGGKTLR 450
ETALEGVDAT AFYPSWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE 500
TGELHPRTLE LLEEVAKRVE QSGIEAWQTL DPRELIGDDA NLYEKNRDTL 550
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASMI 600
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA 650
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD FAQHAVPVDE 700
WLEIDRYAVA FSQQLQTELL GHYEKYEFHP VVAKLQTYCS EDLGGFYLDV 750
LKDRLYTSAA DSRARRSAQT ALYHLTHGLL RVLAPFLSFT AEEAWKVFQP 800
ASDTIYTETY YAYPEVAGSA ALIEKWALLR DVRGNVTKAL EEARTANRIG 850
SSLQAEVAVH ASGARYDALT SLGDDLKFVL ITSAATVVKV DDEAQESVDV 900
AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE IRSAA 945
Length:945
Mass (Da):105,866
Last modified:November 22, 2005 - v1
Checksum:iC6F393E268E23A29
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000151 Genomic DNA. Translation: ABB09438.1.
RefSeqiWP_011352960.1. NC_007510.1.
YP_370082.1. NC_007510.1.

Genome annotation databases

EnsemblBacteriaiABB09438; ABB09438; Bcep18194_A5844.
GeneIDi3751075.
KEGGibur:Bcep18194_A5844.
PATRICi19289189. VBIBurSp120713_3972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000151 Genomic DNA. Translation: ABB09438.1 .
RefSeqi WP_011352960.1. NC_007510.1.
YP_370082.1. NC_007510.1.

3D structure databases

ProteinModelPortali Q39DM8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 269483.Bcep18194_A5844.

Proteomic databases

PRIDEi Q39DM8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB09438 ; ABB09438 ; Bcep18194_A5844 .
GeneIDi 3751075.
KEGGi bur:Bcep18194_A5844.
PATRICi 19289189. VBIBurSp120713_3972.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BSP269483:GHLS-2731-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia sp. 383."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 383.

Entry informationi

Entry nameiSYI_BURS3
AccessioniPrimary (citable) accession number: Q39DM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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