Q39DG2 (T23O_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	Bcep18194_A5910

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Tryptophan 2,3-dioxygenase		PRO_0000360112
Regions
	Region	54 – 58	5	Substrate binding By similarity
	Region	79 – 83	5	Substrate binding By similarity
Sites
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	152	1	Heme By similarity
	Binding site	282	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39DG2 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 02ECD0FC48D81BFB	FASTA	310	35,300
	10 20 30 40 50 60 MQPPGEDAPA GCPFSGARAA HSAPAAPAAH EASHVPGDAG WHNAQLDFSK SMSYGDYLSL 70 80 90 100 110 120 NSILDAQHPL SPDHNEMLFI IQHQTSELWM KLALFELRGA LDAVRTDALP PAFKMLARVS 130 140 150 160 170 180 RILEQLVQAW SVLSTMTPSE YSAMRPYLGQ SSGFQSYQYR QLEFLLGNKN VQMLQPHAHR 190 200 210 220 230 240 PDILEQVRAT LEAPSFYDEV VRLLARRGFP IAAERLERDW TQPTRHDETV EAAWLEVYRH 250 260 270 280 290 300 PQQHWELYEM AEELVDLEDA FRQWRFRHVT TVERIIGFKQ GTGGTSGAPY LRKMLDVVLF 310 PELWHVRTTL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000151 Genomic DNA. Translation: ABB09504.1.
RefSeq	YP_370148.1. NC_007510.1.
3D structure databases
ProteinModelPortal	Q39DG2.
SMR	Q39DG2. Positions 50-310.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39DG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3751141.
GenomeReviews	Gene locus Bcep18194_A5910 in contig CP000151_GR.
KEGG	bur:Bcep18194_A5910.
NMPDR	fig|269483.3.peg.7388.
PATRIC	19289326. VBIBurSp120713_4040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK930429.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_A5910-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURS3

Accession

Primary (citable) accession number: Q39DG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program