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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.UniRule annotation

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei348 – 3481ATPUniRule annotation
Active sitei383 – 3831UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi327 – 3337ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP269483:GHLS-3158-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:Bcep18194_A6269
OrganismiBurkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Isocitrate dehydrogenase kinase/phosphatasePRO_0000259147Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000247673.
KOiK00906.
OrthoDBiPOG091H04UC.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q39CF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHFPKLLSS QIGFDVAQTM LENFDRHYRI FREAAVEAKT LYEHGDWHGL
60 70 80 90 100
QRLARERITS YDDRVKECVE VLEDEYDAEN IDDEVWQQIK LHYIGLLTSH
110 120 130 140 150
RQPECAETFF NSVCCKILHR SYFSNDFIFV RPAISTEYLE NDEPAAKPTY
160 170 180 190 200
RAYYPGTDGL ATTLERIVTN FQLEPAFDDL PRDIGCVMQA IHDEFGHFDE
210 220 230 240 250
APNFQIHVLS SLFFRNKSAY IVGRIINADR VLPFAVPIRH VRPGVLSLDT
260 270 280 290 300
VLLRRDQLMI IFGFSHSYFL VDMGVPSAYV DFLCTIMPGK PKAEIYTSVG
310 320 330 340 350
LQKQGKNLFY RDLLHHLSHS SDRFIIAPGI KGLVMLVFTL PSFPYVFKII
360 370 380 390 400
KDHFPPPKET TRAQIMEKYQ LVKRHDRLGR MADTLEYSSV ALPIARLDHA
410 420 430 440 450
LVRELEKEVP SLLEYEDGNL VIEHLYIERR MTPLNLYLQN GSDSDVEHGV
460 470 480 490 500
KEYGNAVKEL MKANIFPGDM LYKNFGVTRH GRVVFYDYDE IEYLTDCNVR
510 520 530 540 550
RVPPPRNEED ELSGEPWYTV GPHDIFPETY GPFLLGDPRV RDVFMKHHAD
560 570 580 590 600
FFDPALWQAS KDKLIQGELP DFYPYDTALR FCTRYPARFG ATDQNDGAGD

AQRAA
Length:605
Mass (Da):69,925
Last modified:October 31, 2006 - v2
Checksum:i2AE0F8CA247E2CEC
GO

Sequence cautioni

The sequence ABB09863 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB09863.1. Different initiation.
RefSeqiWP_041492972.1. NC_007510.1.

Genome annotation databases

EnsemblBacteriaiABB09863; ABB09863; Bcep18194_A6269.
KEGGibur:Bcep18194_A6269.
PATRICi19290088. VBIBurSp120713_4418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000151 Genomic DNA. Translation: ABB09863.1. Different initiation.
RefSeqiWP_041492972.1. NC_007510.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB09863; ABB09863; Bcep18194_A6269.
KEGGibur:Bcep18194_A6269.
PATRICi19290088. VBIBurSp120713_4418.

Phylogenomic databases

HOGENOMiHOG000247673.
KOiK00906.
OrthoDBiPOG091H04UC.

Enzyme and pathway databases

BioCyciBSP269483:GHLS-3158-MONOMER.

Family and domain databases

HAMAPiMF_00747. AceK. 1 hit.
InterProiIPR010452. Isocitrate_DH_AceK.
[Graphical view]
PfamiPF06315. AceK. 1 hit.
[Graphical view]
PIRSFiPIRSF000719. AceK. 1 hit.
ProDomiPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiACEK_BURL3
AccessioniPrimary (citable) accession number: Q39CF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: September 7, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.