true2009-07-282024-03-27110BIOB_BURL3Complete sequence of chromosome 1 of Burkholderia sp. 383.Copeland A.Lucas S.Lapidus A.Barry K.Detter J.C.Glavina T.Hammon N.Israni S.Pitluck S.Chain P.Malfatti S.Shin M.Vergez L.Schmutz J.Larimer F.Land M.Kyrpides N.Lykidis A.Richardson P.US DOE Joint Genome Institute2005-10EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383Radical_SAMAldolase class IBioBAldolase_TIMBATS_domBiotin_synth/BioABBiotin_synthaseElp3/MiaA/NifB-like_rSAMrSAMbioBBIOTIN SYNTHASEBIOTIN SYNTHASE, MITOCHONDRIALBATSRadical_SAMBiotin_synthbiotin_synthasebiotin_synthase_likeBATSElp3Radical SAM enzymesRADICAL_SAMBiotin synthase2.8.1.6bioBBcep18194_A6278Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.Homodimer.Belongs to the radical SAM superfamily. Biotin synthase family.Biotin synthase370011340Radical SAM core562837175781151462062784Fe-4S-S-AdoMet2005-11-221370012b453c0dce124ab553ab7165bf99c7adMTQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELASAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINNLVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDRALFERLGIRSSDADAMSANAtruetrue