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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotationSAAS annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_02860), Phospho-2-dehydro-3-deoxyheptonate aldolase (WI95_29895), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_14265), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_21645), Phospho-2-dehydro-3-deoxyheptonate aldolase (Bcep18194_A3826), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_13870), Phospho-2-dehydro-3-deoxyheptonate aldolase (Bcep18194_B0086)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK), Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70ShikimateUniRule annotation1
Active sitei74Proton acceptorUniRule annotation1
Binding sitei86NADPUniRule annotation1
Binding sitei95ShikimateUniRule annotation1
Binding sitei110ShikimateUniRule annotation1
Binding sitei226NADP; via carbonyl oxygenUniRule annotation1
Binding sitei228ShikimateUniRule annotation1
Binding sitei249NADP; via carbonyl oxygenUniRule annotation1
Binding sitei256ShikimateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 138NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesisUniRule annotationSAAS annotation
LigandNADPUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Bcep18194_B0345Imported
OrganismiBurkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)Imported
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002705 Componenti: Chromosome 2

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ39AQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 97Shikimate_dh_NInterPro annotationAdd BLAST88

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 20Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000237875.
KOiK00014.
OMAiHEHEGDA.
OrthoDBiPOG091H02DY.

Family and domain databases

HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiView protein in InterPro
IPR036291. NAD(P)-bd_dom_sf.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
PfamiView protein in Pfam
PF08501. Shikimate_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q39AQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPSFLIGL IGQGIGGSLS PAMHEEEGFR QGFGYVYRRI DLDALGVTVD
60 70 80 90 100
ALPQLLDAAQ RMGYNGLNIT HPCKQRVIEH LDALSDDARA LGAVNTVLFK
110 120 130 140 150
DGKRIGHNTD WSGFAKSFRR GLPDASLERV VQLGAGGAGA AVAHAALQMG
160 170 180 190 200
AAELTIFDVD GTRAAALAAE LQQRFPAARV APGGDLASAM RAATGLIHAT
210 220 230 240 250
PTGMAKHPGL PLPAELLHAG MWVADIVYFP LETELIHAAR AAGCATLPGG
260 270 280
GMAVYQAVDA FEIFTGRTPD AERMFDHFQS LVAR
Length:284
Mass (Da):29,965
Last modified:November 22, 2005 - v1
Checksum:iF69CFB036916D808
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000152 Genomic DNA. Translation: ABB10461.1.
RefSeqiWP_011353959.1. NC_007511.1.

Genome annotation databases

EnsemblBacteriaiABB10461; ABB10461; Bcep18194_B0345.
KEGGibur:Bcep18194_B0345.
PATRICifig|482957.22.peg.3930.

Similar proteinsi

Entry informationi

Entry nameiQ39AQ0_BURL3
AccessioniPrimary (citable) accession number: Q39AQ0
Entry historyiIntegrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 25, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported