Reviewed, UniProtKB/Swiss-Prot Q39AN3 (METE_BURS3)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	Bcep18194_B0362

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017232
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39AN3-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 62C3FB0D591620BE	FASTA	764	85,124
	10 20 30 40 50 60 MVTTHNLGFP RIGAKRELKF GLERYWKGES SRGALKALGA ELRQRHWNDQ RDLDLAPVGD 70 80 90 100 110 120 FAFYDQVLDM SFTLGNLPKR VQGFHGDALD NYFRVARGRS AQSAEEHAAC CGGVSAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSRLLQQ LAEAQAQGVA AKPVIVGPVT YLWLGKAKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAAWQQAFRT AYAALETRRI 250 260 270 280 290 300 KVLLATYFGQ LQGNLALATS LPVDGLHVDA INARDEVDAL ARELPAERVL SVGAINGRNI 310 320 330 340 350 360 WKTDLNATLD WLEPLAKRLG DRLWLAPSCS LLHVPVDLAS EEKLDAEIRS WLAFALQKLD 370 380 390 400 410 420 ELKVLATALN EGRDKVADAL AANAAAIHSR RHSPRVNNPA VKAAIARIDA QLGNRVSPYT 430 440 450 460 470 480 QRAPKQSARL NLPAFPTTTI GSFPQTGEIR QARSQFKAGT LDEAGYRKAM QAEIERSVRE 490 500 510 520 530 540 QESLELDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWITYAQ SLTNKPMKGM LTGPVTILNW SFVRDDQPRS VSCYQLALAI REEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWGEYLKWA VESFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDNFK YPNEIGPGVY DIHSPNIPTQ EHIVGLMKKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWAEVIPALT NMVAAAKTLR NQVQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000152 Genomic DNA. Translation: ABB10478.1.
RefSeq	YP_371122.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q39AN3.
Genome annotation databases
GeneID	3752126.
GenomeReviews	Gene locus Bcep18194_B0362 in contig CP000152_GR.
KEGG	bur:Bcep18194_B0362.
NMPDR	fig|269483.3.peg.364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q39AN3.
OMA	CSLLHTP.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B0362-MON.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURS3

Accession

Primary (citable) accession number: Q39AN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)