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Q39AM3 (NADE_BURS3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NH(3)-dependent NAD(+) synthetase

EC=6.3.1.5
Gene names
Name:nadE
Ordered Locus Names:Bcep18194_B0372
OrganismBurkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]
Taxonomic identifier269483 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193

Sequence similarities

Belongs to the NAD synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
PRO_1000099014

Regions

Nucleotide binding51 – 588ATP By similarity

Sites

Active site531 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q39AM3 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 405CA09F0D6180D8

FASTA28230,798
        10         20         30         40         50         60 
MTSADYASRQ RAIIAELNVA SEFDAEAEIA RRVEFLAQYL RSTGLRTYVL GISGGVDSST 

        70         80         90        100        110        120 
AGRLAQLSVE KLRADGYDAR FVAMRLPNGV QNDEADAQRA LAFVRADEEL TVNVKPAADA 

       130        140        150        160        170        180 
MLGALAASGH AFETPAQQDF VHGNIKARER MIAQYAVAGA RRGIVIGTDH AAESLMGFFT 

       190        200        210        220        230        240 
KFGDGGADIL PLAGLNKRRV RAVARALGGE ELIVMKVPTA DLEELRPLRP DEHAYGVSYD 

       250        260        270        280 
EIDDFLEGKP VADNVYETVL RFYDASRHKR ALPYTMFDWP TA 

« Hide

References

[1]"Complete sequence of chromosome 2 of Burkholderia sp. 383."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 383.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000152 Genomic DNA. Translation: ABB10488.1.
RefSeqYP_371132.1. NC_007511.1.

3D structure databases

HSSPHSSP built from PDB template 1WXH based on UniProtKB P18843.
ProteinModelPortalQ39AM3.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ39AM3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3752136.
GenomeReviewsGene locus Bcep18194_B0372 in contig CP000152_GR.
KEGGbur:Bcep18194_B0372.
NMPDRfig|269483.3.peg.374.
PATRIC19291426. VBIBurSp120713_5078.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0171.
HOGENOMHBG351567.
OMAGLNKRRV.
ProtClustDBPRK00768.

Enzyme and pathway databases

BioCycBSP36773:BCEP18194_B0372-MONOMER.

Family and domain databases

HAMAPMF_00193. NadE.
[Tree]
InterProIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01916.
PfamPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00552. NadE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADE_BURS3
AccessionPrimary (citable) accession number: Q39AM3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 22, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families