Q39AM3 (NADE_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Bcep18194_B0372

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099014
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39AM3 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 405CA09F0D6180D8	FASTA	282	30,798
	10 20 30 40 50 60 MTSADYASRQ RAIIAELNVA SEFDAEAEIA RRVEFLAQYL RSTGLRTYVL GISGGVDSST 70 80 90 100 110 120 AGRLAQLSVE KLRADGYDAR FVAMRLPNGV QNDEADAQRA LAFVRADEEL TVNVKPAADA 130 140 150 160 170 180 MLGALAASGH AFETPAQQDF VHGNIKARER MIAQYAVAGA RRGIVIGTDH AAESLMGFFT 190 200 210 220 230 240 KFGDGGADIL PLAGLNKRRV RAVARALGGE ELIVMKVPTA DLEELRPLRP DEHAYGVSYD 250 260 270 280 EIDDFLEGKP VADNVYETVL RFYDASRHKR ALPYTMFDWP TA

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000152 Genomic DNA. Translation: ABB10488.1.
RefSeq	YP_371132.1. NC_007511.1.
3D structure databases
HSSP	HSSP built from PDB template 1WXH based on UniProtKB P18843.
ProteinModelPortal	Q39AM3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39AM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3752136.
GenomeReviews	Gene locus Bcep18194_B0372 in contig CP000152_GR.
KEGG	bur:Bcep18194_B0372.
NMPDR	fig|269483.3.peg.374.
PATRIC	19291426. VBIBurSp120713_5078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B0372-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURS3

Accession

Primary (citable) accession number: Q39AM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program