Q39A07 (SYC1_BURS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase 1
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase 1
Short name=CysRS 1

Gene names

Name:	cysS1
Ordered Locus Names:	Bcep18194_B0590

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Cysteine--tRNA ligase 1 HAMAP MF_00041		PRO_0000240896
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	279 – 283	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	221	1	Zinc By similarity
	Metal binding	246	1	Zinc By similarity
	Metal binding	250	1	Zinc By similarity
	Binding site	282	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39A07 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E35C2FB5681517B4	FASTA	470	53,285
	10 20 30 40 50 60 MPLALYDTWS RTVRPFTPIR AGQVGMYGCG PTVYDDAHIG NLRTYVFEDL LRRVLERNGY 70 80 90 100 110 120 AVRHVVNITD VGHLTSDADE GEDKMEKGSR RTGESAWAIA RRYTEAFVRD WHALNLLEPT 130 140 150 160 170 180 IWCRATDHIA EQIAFIETLD RAGYVYRTDD GLYFDTSRQD DYGYLARLDR AGLQAGKRVA 190 200 210 220 230 240 LGGKRSITDF ALWKFSPADV QRQMEWDSPW GRGFPGWHIE CSAMSAKYLG TLFDIHCGGE 250 260 270 280 290 300 DHIAVHHSNE IAQTRAAHGT QLANYWMHGH FLTLDVHTKM SKSSGDFVRL QTLQRRGVDP 310 320 330 340 350 360 LAYRYLCLTA HYRSKLHFTW ESLDAAQTAL NRLRHLYIGW PDGGRVDADF AARFDAEVNE 370 380 390 400 410 420 DLNLPRALAV LWDLVRSNLP PATLKATVDS FDMVLGLGLR EWRPVAVDVP EHVRALLGER 430 440 450 460 470 ARARAEKDWA QADRIREALS AEGWRVEDTP EGQRLFGMAM GASEPDVPPQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000152 Genomic DNA. Translation: ABB10704.1.
RefSeq	YP_371348.1. NC_007511.1.
3D structure databases
ProteinModelPortal	Q39A07.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39A07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3752354.
GenomeReviews	Gene locus Bcep18194_B0590 in contig CP000152_GR.
KEGG	bur:Bcep18194_B0590.
NMPDR	fig|269483.3.peg.1010.
PATRIC	19291897. VBIBurSp120713_5313.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	IGNLRTY.
ProtClustDB	CLSK932133.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B0590-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_BURS3

Accession

Primary (citable) accession number: Q39A07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program