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Protein

Vetispiradiene synthase 2

Gene
N/A
Organism
Hyoscyamus muticus (Egyptian henbane)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sesquiterpene synthase that catalyzes the formation of vetispiradiene from trans,trans-farnesyl diphosphate. The initial internal cyclization produces the monocyclic intermediate germacrene A.

Catalytic activityi

(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathway:iterpenoid biosynthesis

This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Magnesium 1By similarity
Metal bindingi54 – 541Magnesium 2By similarity
Metal bindingi58 – 581Magnesium 1By similarity
Metal bindingi58 – 581Magnesium 2By similarity
Metal bindingi197 – 1971Magnesium 3By similarity
Metal bindingi201 – 2011Magnesium 3By similarity
Metal bindingi205 – 2051Magnesium 3By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
Vetispiradiene synthase 2 (EC:4.2.3.21)
Short name:
HVS2
OrganismiHyoscyamus muticus (Egyptian henbane)
Taxonomic identifieri35626 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeHyoscyameaeHyoscyamus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 300›300Vetispiradiene synthase 2PRO_0000398185Add
BLAST

Expressioni

Inductioni

By elicitor from R.solani.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ39979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi54 – 585DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsa subfamily.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR005630. Terpene_synthase_metal-bd.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q39979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SRWWKDLDFV TTLPYARDRA VECYFWTMGV YAEPQYSQAR VMLAKTIAMI
60 70 80 90 100
SIVDDTFDAY GIVKEFEVYT DAIQRWDISQ IDRLPEYMKI SYKALLDLYD
110 120 130 140 150
DYEKELSKDG RSDVVHYAKE RMKEIVRNYF IEAKWFIEGY MPSVSEYLSN
160 170 180 190 200
ALATSTYYLL TTTSYLGMKS ATKEHFEWLA TNPKILEANA TLCRVVDDIA
210 220 230 240 250
TYEVEKGRGQ IATGIECYMR DYGVSTEVAM EKFQEMAEIA WKDVNEEILR
260 270 280 290 300
PTPVSAEILT RILNLARIID VTYKHNQDGY THPEKFKPHI IALLVDSIEI
Length:300
Mass (Da):34,959
Last modified:November 1, 1996 - v1
Checksum:i9F60D2BAB580A79D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20189 mRNA. Translation: AAA86338.1.
PIRiB56118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20189 mRNA. Translation: AAA86338.1.
PIRiB56118.

3D structure databases

ProteinModelPortaliQ39979.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00213.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR005630. Terpene_synthase_metal-bd.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases."
    Back K., Chappell J.
    J. Biol. Chem. 270:7375-7381(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION BY ELICITOR.

Entry informationi

Entry nameiVTSS2_HYOMU
AccessioniPrimary (citable) accession number: Q39979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.