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Q39979 (VTSS2_HYOMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vetispiradiene synthase 2

Short name=HVS2
EC=4.2.3.21
OrganismHyoscyamus muticus (Egyptian henbane)
Taxonomic identifier35626 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeHyoscyameaeHyoscyamus

Protein attributes

Sequence length300 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sesquiterpene synthase that catalyzes the formation of vetispiradiene from trans,trans-farnesyl diphosphate. The initial internal cyclization produces the monocyclic intermediate germacrene A.

Catalytic activity

(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate. Ref.1

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Subcellular location

Cytoplasm Probable.

Induction

By elicitor from R.solani. Ref.1

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family. Tpsa subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processterpenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

vetispiradiene synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 300›300Vetispiradiene synthase 2
PRO_0000398185

Regions

Motif54 – 585DDXXD motif

Sites

Metal binding541Magnesium 1 By similarity
Metal binding541Magnesium 2 By similarity
Metal binding581Magnesium 1 By similarity
Metal binding581Magnesium 2 By similarity
Metal binding1971Magnesium 3 By similarity
Metal binding2011Magnesium 3 By similarity
Metal binding2051Magnesium 3 By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q39979 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9F60D2BAB580A79D

FASTA30034,959
        10         20         30         40         50         60 
SRWWKDLDFV TTLPYARDRA VECYFWTMGV YAEPQYSQAR VMLAKTIAMI SIVDDTFDAY 

        70         80         90        100        110        120 
GIVKEFEVYT DAIQRWDISQ IDRLPEYMKI SYKALLDLYD DYEKELSKDG RSDVVHYAKE 

       130        140        150        160        170        180 
RMKEIVRNYF IEAKWFIEGY MPSVSEYLSN ALATSTYYLL TTTSYLGMKS ATKEHFEWLA 

       190        200        210        220        230        240 
TNPKILEANA TLCRVVDDIA TYEVEKGRGQ IATGIECYMR DYGVSTEVAM EKFQEMAEIA 

       250        260        270        280        290        300 
WKDVNEEILR PTPVSAEILT RILNLARIID VTYKHNQDGY THPEKFKPHI IALLVDSIEI 

« Hide

References

[1]"Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases."
Back K., Chappell J.
J. Biol. Chem. 270:7375-7381(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION BY ELICITOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20189 mRNA. Translation: AAA86338.1.
PIRB56118.

3D structure databases

ProteinModelPortalQ39979.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00213.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVTSS2_HYOMU
AccessionPrimary (citable) accession number: Q39979
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways