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Q39979

- VTSS2_HYOMU

UniProt

Q39979 - VTSS2_HYOMU

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Protein

Vetispiradiene synthase 2

Gene
N/A
Organism
Hyoscyamus muticus (Egyptian henbane)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Sesquiterpene synthase that catalyzes the formation of vetispiradiene from trans,trans-farnesyl diphosphate. The initial internal cyclization produces the monocyclic intermediate germacrene A.

Catalytic activityi

(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate.1 Publication

Cofactori

Binds 3 magnesium ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Magnesium 1By similarity
Metal bindingi54 – 541Magnesium 2By similarity
Metal bindingi58 – 581Magnesium 1By similarity
Metal bindingi58 – 581Magnesium 2By similarity
Metal bindingi197 – 1971Magnesium 3By similarity
Metal bindingi201 – 2011Magnesium 3By similarity
Metal bindingi205 – 2051Magnesium 3By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. vetispiradiene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. terpenoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
Vetispiradiene synthase 2 (EC:4.2.3.21)
Short name:
HVS2
OrganismiHyoscyamus muticus (Egyptian henbane)
Taxonomic identifieri35626 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeHyoscyameaeHyoscyamus

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 300›300Vetispiradiene synthase 2PRO_0000398185Add
BLAST

Expressioni

Inductioni

By elicitor from R.solani.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ39979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi54 – 585DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsa subfamily.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q39979 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
SRWWKDLDFV TTLPYARDRA VECYFWTMGV YAEPQYSQAR VMLAKTIAMI
60 70 80 90 100
SIVDDTFDAY GIVKEFEVYT DAIQRWDISQ IDRLPEYMKI SYKALLDLYD
110 120 130 140 150
DYEKELSKDG RSDVVHYAKE RMKEIVRNYF IEAKWFIEGY MPSVSEYLSN
160 170 180 190 200
ALATSTYYLL TTTSYLGMKS ATKEHFEWLA TNPKILEANA TLCRVVDDIA
210 220 230 240 250
TYEVEKGRGQ IATGIECYMR DYGVSTEVAM EKFQEMAEIA WKDVNEEILR
260 270 280 290 300
PTPVSAEILT RILNLARIID VTYKHNQDGY THPEKFKPHI IALLVDSIEI
Length:300
Mass (Da):34,959
Last modified:November 1, 1996 - v1
Checksum:i9F60D2BAB580A79D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20189 mRNA. Translation: AAA86338.1.
PIRiB56118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20189 mRNA. Translation: AAA86338.1 .
PIRi B56118.

3D structure databases

ProteinModelPortali Q39979.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00213 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases."
    Back K., Chappell J.
    J. Biol. Chem. 270:7375-7381(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION BY ELICITOR.

Entry informationi

Entry nameiVTSS2_HYOMU
AccessioniPrimary (citable) accession number: Q39979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3