Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q39979

- VTSS2_HYOMU

UniProt

Q39979 - VTSS2_HYOMU

Protein

Vetispiradiene synthase 2

Gene
N/A
Organism
Hyoscyamus muticus (Egyptian henbane)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sesquiterpene synthase that catalyzes the formation of vetispiradiene from trans,trans-farnesyl diphosphate. The initial internal cyclization produces the monocyclic intermediate germacrene A.

    Catalytic activityi

    (2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate.1 Publication

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Magnesium 1By similarity
    Metal bindingi54 – 541Magnesium 2By similarity
    Metal bindingi58 – 581Magnesium 1By similarity
    Metal bindingi58 – 581Magnesium 2By similarity
    Metal bindingi197 – 1971Magnesium 3By similarity
    Metal bindingi201 – 2011Magnesium 3By similarity
    Metal bindingi205 – 2051Magnesium 3By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. vetispiradiene synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. terpenoid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00213.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vetispiradiene synthase 2 (EC:4.2.3.21)
    Short name:
    HVS2
    OrganismiHyoscyamus muticus (Egyptian henbane)
    Taxonomic identifieri35626 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeHyoscyameaeHyoscyamus

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 300›300Vetispiradiene synthase 2PRO_0000398185Add
    BLAST

    Expressioni

    Inductioni

    By elicitor from R.solani.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ39979.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi54 – 585DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family. Tpsa subfamily.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR005630. Terpene_synthase_metal-bd.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Q39979-1 [UniParc]FASTAAdd to Basket

    « Hide

    SRWWKDLDFV TTLPYARDRA VECYFWTMGV YAEPQYSQAR VMLAKTIAMI    50
    SIVDDTFDAY GIVKEFEVYT DAIQRWDISQ IDRLPEYMKI SYKALLDLYD 100
    DYEKELSKDG RSDVVHYAKE RMKEIVRNYF IEAKWFIEGY MPSVSEYLSN 150
    ALATSTYYLL TTTSYLGMKS ATKEHFEWLA TNPKILEANA TLCRVVDDIA 200
    TYEVEKGRGQ IATGIECYMR DYGVSTEVAM EKFQEMAEIA WKDVNEEILR 250
    PTPVSAEILT RILNLARIID VTYKHNQDGY THPEKFKPHI IALLVDSIEI 300
    Length:300
    Mass (Da):34,959
    Last modified:November 1, 1996 - v1
    Checksum:i9F60D2BAB580A79D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20189 mRNA. Translation: AAA86338.1.
    PIRiB56118.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20189 mRNA. Translation: AAA86338.1 .
    PIRi B56118.

    3D structure databases

    ProteinModelPortali Q39979.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00213 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR005630. Terpene_synthase_metal-bd.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases."
      Back K., Chappell J.
      J. Biol. Chem. 270:7375-7381(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION BY ELICITOR.

    Entry informationi

    Entry nameiVTSS2_HYOMU
    AccessioniPrimary (citable) accession number: Q39979
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3