Q39857 (XTH_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase
EC=2.4.1.207

Gene names

Name:	XTH
Synonyms:	EXT

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	295 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 22	›22	Potential
	Chain	23 – 295	273	Probable xyloglucan endotransglucosylase/hydrolase		PRO_0000011839
Sites
	Active site	107	1	Nucleophile By similarity
	Active site	111	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q39857 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B34DC5015145DE0D	FASTA	295	34,416
	10 20 30 40 50 60 IPVFSVWTVC VILASLASAA LCANPRRPVD VQFGRNYVPT WAFDHIKYFN GGSDIQLHLD 70 80 90 100 110 120 KYTGTGFQSK GSYLFGHFSM YIKMVPGDSA GTVTAFYLSS QNAEHDEIDF EFLGNRTGQP 130 140 150 160 170 180 YILQTNVFTG GKGDREQRIY LWFDPTKEYH RYSILWNLYQ IVFFVDEVPI RVFKNSKDLG 190 200 210 220 230 240 VKFPFDQPMK IYNSLWNADD WATRGGLEKT DWSKAPFIAA YKGFHIDGCE ASVNAKFCDT 250 260 270 280 290 QGKRWWDQPE FRDLDAAQWR RLRWVRQKYT IYNYCTDTKR YPHISPPECK RDRDI

References

[1]

"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls." Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K. J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16455 mRNA. Translation: BAA03922.1.
PIR	B49539.
UniGene	Gma.2240.
3D structure databases
ProteinModelPortal	Q39857.
SMR	Q39857. Positions 28-295.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Gene expression databases
Genevestigator	Q39857.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH_SOYBN

Accession

Primary (citable) accession number: Q39857

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program