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Q39857 (XTH_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase

EC=2.4.1.207
Gene names
Name:XTH
Synonyms:EXT
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length295 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide‹1 – 22›22 Potential
Chain23 – 295273Probable xyloglucan endotransglucosylase/hydrolase
PRO_0000011839

Sites

Active site1071Nucleophile By similarity
Active site1111Proton donor By similarity

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) Potential

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q39857 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B34DC5015145DE0D

FASTA29534,416
        10         20         30         40         50         60 
IPVFSVWTVC VILASLASAA LCANPRRPVD VQFGRNYVPT WAFDHIKYFN GGSDIQLHLD 

        70         80         90        100        110        120 
KYTGTGFQSK GSYLFGHFSM YIKMVPGDSA GTVTAFYLSS QNAEHDEIDF EFLGNRTGQP 

       130        140        150        160        170        180 
YILQTNVFTG GKGDREQRIY LWFDPTKEYH RYSILWNLYQ IVFFVDEVPI RVFKNSKDLG 

       190        200        210        220        230        240 
VKFPFDQPMK IYNSLWNADD WATRGGLEKT DWSKAPFIAA YKGFHIDGCE ASVNAKFCDT 

       250        260        270        280        290 
QGKRWWDQPE FRDLDAAQWR RLRWVRQKYT IYNYCTDTKR YPHISPPECK RDRDI 

« Hide

References

[1]"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16455 mRNA. Translation: BAA03922.1.
PIRB49539.
UniGeneGma.2240.

3D structure databases

ProteinModelPortalQ39857.
SMRQ39857. Positions 28-295.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Gene expression databases

GenevestigatorQ39857.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH_SOYBN
AccessionPrimary (citable) accession number: Q39857
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries