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Q39857

- XTH_SOYBN

UniProt

Q39857 - XTH_SOYBN

Protein

Probable xyloglucan endotransglucosylase/hydrolase

Gene

XTH

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei107 – 1071NucleophilePROSITE-ProRule annotation
    Active sitei111 – 1111Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase (EC:2.4.1.207)
    Gene namesi
    Name:XTH
    Synonyms:EXT
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 22›22Sequence AnalysisAdd
    BLAST
    Chaini23 – 295273Probable xyloglucan endotransglucosylase/hydrolasePRO_0000011839Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriQ39857.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39857.
    SMRiQ39857. Positions 28-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q39857-1 [UniParc]FASTAAdd to Basket

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    IPVFSVWTVC VILASLASAA LCANPRRPVD VQFGRNYVPT WAFDHIKYFN    50
    GGSDIQLHLD KYTGTGFQSK GSYLFGHFSM YIKMVPGDSA GTVTAFYLSS 100
    QNAEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEYH 150
    RYSILWNLYQ IVFFVDEVPI RVFKNSKDLG VKFPFDQPMK IYNSLWNADD 200
    WATRGGLEKT DWSKAPFIAA YKGFHIDGCE ASVNAKFCDT QGKRWWDQPE 250
    FRDLDAAQWR RLRWVRQKYT IYNYCTDTKR YPHISPPECK RDRDI 295
    Length:295
    Mass (Da):34,416
    Last modified:November 1, 1996 - v1
    Checksum:iB34DC5015145DE0D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16455 mRNA. Translation: BAA03922.1.
    PIRiB49539.
    UniGeneiGma.2240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16455 mRNA. Translation: BAA03922.1 .
    PIRi B49539.
    UniGenei Gma.2240.

    3D structure databases

    ProteinModelPortali Q39857.
    SMRi Q39857. Positions 28-295.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Gene expression databases

    Genevestigatori Q39857.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
      Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
      J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiXTH_SOYBN
    AccessioniPrimary (citable) accession number: Q39857
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3