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Q39857

- XTH_SOYBN

UniProt

Q39857 - XTH_SOYBN

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Protein

Probable xyloglucan endotransglucosylase/hydrolase

Gene

XTH

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071NucleophilePROSITE-ProRule annotation
Active sitei111 – 1111Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase (EC:2.4.1.207)
Gene namesi
Name:XTH
Synonyms:EXT
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 22›22Sequence AnalysisAdd
BLAST
Chaini23 – 295273Probable xyloglucan endotransglucosylase/hydrolasePRO_0000011839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriQ39857.

Structurei

3D structure databases

ProteinModelPortaliQ39857.
SMRiQ39857. Positions 28-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ39857.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39857-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
IPVFSVWTVC VILASLASAA LCANPRRPVD VQFGRNYVPT WAFDHIKYFN
60 70 80 90 100
GGSDIQLHLD KYTGTGFQSK GSYLFGHFSM YIKMVPGDSA GTVTAFYLSS
110 120 130 140 150
QNAEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEYH
160 170 180 190 200
RYSILWNLYQ IVFFVDEVPI RVFKNSKDLG VKFPFDQPMK IYNSLWNADD
210 220 230 240 250
WATRGGLEKT DWSKAPFIAA YKGFHIDGCE ASVNAKFCDT QGKRWWDQPE
260 270 280 290
FRDLDAAQWR RLRWVRQKYT IYNYCTDTKR YPHISPPECK RDRDI
Length:295
Mass (Da):34,416
Last modified:November 1, 1996 - v1
Checksum:iB34DC5015145DE0D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16455 mRNA. Translation: BAA03922.1.
PIRiB49539.
UniGeneiGma.2240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16455 mRNA. Translation: BAA03922.1 .
PIRi B49539.
UniGenei Gma.2240.

3D structure databases

ProteinModelPortali Q39857.
SMRi Q39857. Positions 28-295.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

InParanoidi Q39857.

Gene expression databases

Genevestigatori Q39857.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
    Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
    J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiXTH_SOYBN
AccessioniPrimary (citable) accession number: Q39857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3