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Protein

Xyloglucan endotransglucosylase/hydrolase 1

Gene

XTH1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081NucleophilePROSITE-ProRule annotation
Active sitei112 – 1121Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase 1Curated (EC:2.4.1.207By similarity)
Gene namesi
Name:XTH1Curated
Synonyms:EXT1 Publication, XTHCurated
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 296273Xyloglucan endotransglucosylase/hydrolase 1CuratedPRO_0000011839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
N-glycosylated; not essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi3847.GLYMA16G04950.1.

Structurei

3D structure databases

ProteinModelPortaliQ39857.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ39857.
KOiK08235.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSSSMWTV CVILASLASA ALCANPRRPV DVQFGRNYVP TWAFDHIKYF
60 70 80 90 100
NGGSDIQPHL DKYTGTGFQP KGSYLFGHFS MYIKMVPGDS AGTVTAFYLS
110 120 130 140 150
SQNAEHDEID FEFLGNRTGQ PYILQTNVFT GGKGDREQRI YLWFDPTKEY
160 170 180 190 200
HRYSILWNLY QIVFFVDEVP IRVFKNSKDL GVKFPFDQPM KIYNSLWNAD
210 220 230 240 250
DWATRGGLEK TDWSKAPFIA AYKGFHIDGC EASVNAKFCD TQGKRWWDQP
260 270 280 290
EFRDLDAAQW RRLRWVRQKY TIYNYCTDTK RYPHISPPEC KRDRDI
Length:296
Mass (Da):34,435
Last modified:January 7, 2015 - v2
Checksum:i9BF579B92A56D07F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 54GSSS → IPVF in BAA03922 (PubMed:8244968).Curated
Sequence conflicti7 – 71M → V in BAA03922 (PubMed:8244968).Curated
Sequence conflicti58 – 581P → L in BAA03922 (PubMed:8244968).Curated
Sequence conflicti70 – 701P → S in BAA03922 (PubMed:8244968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT098780 mRNA. Translation: ACU23972.1.
D16455 mRNA. Translation: BAA03922.1.
PIRiB49539.
RefSeqiNP_001242655.1. NM_001255726.2.
UniGeneiGma.2240.

Genome annotation databases

GeneIDi547775.
KEGGigmx:547775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT098780 mRNA. Translation: ACU23972.1.
D16455 mRNA. Translation: BAA03922.1.
PIRiB49539.
RefSeqiNP_001242655.1. NM_001255726.2.
UniGeneiGma.2240.

3D structure databases

ProteinModelPortaliQ39857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA16G04950.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi547775.
KEGGigmx:547775.

Phylogenomic databases

InParanoidiQ39857.
KOiK08235.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cheung F., Xiao Y., Chan A., Moskal W., Town C.D.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
    Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
    J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-296.

Entry informationi

Entry nameiXTH1_SOYBN
AccessioniPrimary (citable) accession number: Q39857
Secondary accession number(s): C6TM30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: January 7, 2015
Last modified: July 22, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.