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Protein

Dynamin-related protein 12A

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein that is targeted to the forming cell plate during cytokinesis. May be involved in attaching Golgi-derived vesicles to microtubules which direct vesicles to the forming cell plate during cytokinesis. Possesses intrinsic GTPase activity in vitro.3 Publications

Miscellaneous

The term phragmoplastin refers to the location of the protein to the phragmoplast across the whole width of the newly formed cell plate, unlike phragmoplast microtubules which are concentrated on the periphery of the forming plate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 48GTPBy similarity8
Nucleotide bindingi142 – 146GTPBy similarity5
Nucleotide bindingi211 – 214GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Motor protein
Biological processCell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-related protein 12A
Alternative name(s):
Phragmoplastin
Soybean dynamin-like protein 12A
Short name:
SDL12A
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47K → M: Dominant negative. Loss of GTPase activity and reduced transport of vesicles to the cell plate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003345861 – 610Dynamin-related protein 12AAdd BLAST610

Proteomic databases

PRIDEiQ39821

Expressioni

Tissue specificityi

Expressed in seedling root tips and root elongation zones, young root nodules and young leaves.1 Publication

Interactioni

Subunit structurei

May homooligomerize and heterooligomerize. May interact with CALS1 and UGT1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UGT75B1Q9LR442EBI-1765815,EBI-1765823From Arabidopsis thaliana.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ39821, 2 interactors
STRINGi3847.GLYMA05G36840.1

Structurei

3D structure databases

ProteinModelPortaliQ39821
SMRiQ39821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 300Dynamin-type GPROSITE-ProRule annotationAdd BLAST270
Domaini518 – 610GEDPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 48G1 motifPROSITE-ProRule annotation8
Regioni67 – 69G2 motifPROSITE-ProRule annotation3
Regioni142 – 145G3 motifPROSITE-ProRule annotation4
Regioni211 – 214G4 motifPROSITE-ProRule annotation4
Regioni241 – 244G5 motifPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446 Eukaryota
COG0699 LUCA

Family and domain databases

CDDicd08771 DLP_1, 1 hit
InterProiView protein in InterPro
IPR000375 Dynamin_central
IPR001401 Dynamin_GTPase
IPR019762 Dynamin_GTPase_CS
IPR022812 Dynamin_SF
IPR030381 G_DYNAMIN_dom
IPR003130 GED
IPR020850 GED_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11566 PTHR11566, 1 hit
PfamiView protein in Pfam
PF01031 Dynamin_M, 1 hit
PF00350 Dynamin_N, 1 hit
PF02212 GED, 1 hit
PRINTSiPR00195 DYNAMIN
SMARTiView protein in SMART
SM00053 DYNc, 1 hit
SM00302 GED, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00410 G_DYNAMIN_1, 1 hit
PS51718 G_DYNAMIN_2, 1 hit
PS51388 GED, 1 hit

Sequencei

Sequence statusi: Complete.

Q39821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLISLVNK IQRACTALGD HGENSALPTL WDSLPAIAVV GGQSSGKSSV
60 70 80 90 100
LESVVGKDFL PRGSGIVTRR PLVLQLHKID EGSREYAEFL HLPRKRFTDF
110 120 130 140 150
VAVRKEIQDE TDRETGRTKQ ISSVPIHLSI YSPNVVNLTL IDLPGLTKVA
160 170 180 190 200
VEGQPDSIVK DIEDMVRSYI EKPNCIILAI SPANQDLATS DAIKISREVD
210 220 230 240 250
PTGDRTIGVL TKIDLMDKGT DAVDILEGRA YRLKFPWIGV VNRSQQDINK
260 270 280 290 300
NVDMIAARRR EREYFNSTPE YKHLANRMGS EHLAKMLSKH LETVIKSKIP
310 320 330 340 350
GIQSLINKTI AELEAELTRL GKPVAADAGG KLYAIMEICR SFDQIFKDHL
360 370 380 390 400
DGVRPGGDKI YNVFDNQLPA ALKRLQFDKQ LSMENIRKLI TEADGYQPHL
410 420 430 440 450
IAPEQGYRRL IESSLITIRG PAESAVDAVH SLLKDLVHKA MSETLDLKQY
460 470 480 490 500
PGLRVEVGAA SVDSLERMRD ESKRATLQLV DMECGYLTVD FFRKLPQDVD
510 520 530 540 550
KGGNPTHSIC DRYNDSYLRR IGTTILSYVN MVCATLRHSI PKSIVYCQVR
560 570 580 590 600
EAKRSLLDHF FTELGKMEIK RLSSLLNEDP AIMERRSALA KRLELYRSAQ
610
AEIDAVAWSK
Length:610
Mass (Da):68,356
Last modified:November 1, 1996 - v1
Checksum:i62AF611E6A615F15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25547 mRNA Translation: AAB05992.1
PIRiS63667
RefSeqiNP_001235175.1, NM_001248246.1
UniGeneiGma.33453

Genome annotation databases

GeneIDi547855
KEGGigmx:547855

Similar proteinsi

Entry informationi

Entry nameiSDLCA_SOYBN
AccessioniPrimary (citable) accession number: Q39821
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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