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Protein

Dynamin-related protein 12A

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein that is targeted to the forming cell plate during cytokinesis. May be involved in attaching Golgi-derived vesicles to microtubules which direct vesicles to the forming cell plate during cytokinesis. Possesses intrinsic GTPase activity in vitro.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 488GTPBy similarity
Nucleotide bindingi142 – 1465GTPBy similarity
Nucleotide bindingi211 – 2144GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-related protein 12A
Alternative name(s):
Phragmoplastin
Soybean dynamin-like protein 12A
Short name:
SDL12A
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytoskeletonphragmoplast 1 Publication

  • Note: Localized in the forming cell plate during cytokinesis (at protein level).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471K → M: Dominant negative. Loss of GTPase activity and reduced transport of vesicles to the cell plate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Dynamin-related protein 12APRO_0000334586Add
BLAST

Expressioni

Tissue specificityi

Expressed in seedling root tips and root elongation zones, young root nodules and young leaves.1 Publication

Interactioni

Subunit structurei

May homooligomerize and heterooligomerize. May interact with CALS1 and UGT1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UGT75B1Q9LR442EBI-1765815,EBI-1765823From a different organism.

Protein-protein interaction databases

IntActiQ39821. 2 interactions.
STRINGi3847.GLYMA05G36840.1.

Structurei

3D structure databases

ProteinModelPortaliQ39821.
SMRiQ39821. Positions 1-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 300270Dynamin-type GAdd
BLAST
Domaini518 – 61093GEDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q39821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLISLVNK IQRACTALGD HGENSALPTL WDSLPAIAVV GGQSSGKSSV
60 70 80 90 100
LESVVGKDFL PRGSGIVTRR PLVLQLHKID EGSREYAEFL HLPRKRFTDF
110 120 130 140 150
VAVRKEIQDE TDRETGRTKQ ISSVPIHLSI YSPNVVNLTL IDLPGLTKVA
160 170 180 190 200
VEGQPDSIVK DIEDMVRSYI EKPNCIILAI SPANQDLATS DAIKISREVD
210 220 230 240 250
PTGDRTIGVL TKIDLMDKGT DAVDILEGRA YRLKFPWIGV VNRSQQDINK
260 270 280 290 300
NVDMIAARRR EREYFNSTPE YKHLANRMGS EHLAKMLSKH LETVIKSKIP
310 320 330 340 350
GIQSLINKTI AELEAELTRL GKPVAADAGG KLYAIMEICR SFDQIFKDHL
360 370 380 390 400
DGVRPGGDKI YNVFDNQLPA ALKRLQFDKQ LSMENIRKLI TEADGYQPHL
410 420 430 440 450
IAPEQGYRRL IESSLITIRG PAESAVDAVH SLLKDLVHKA MSETLDLKQY
460 470 480 490 500
PGLRVEVGAA SVDSLERMRD ESKRATLQLV DMECGYLTVD FFRKLPQDVD
510 520 530 540 550
KGGNPTHSIC DRYNDSYLRR IGTTILSYVN MVCATLRHSI PKSIVYCQVR
560 570 580 590 600
EAKRSLLDHF FTELGKMEIK RLSSLLNEDP AIMERRSALA KRLELYRSAQ
610
AEIDAVAWSK
Length:610
Mass (Da):68,356
Last modified:November 1, 1996 - v1
Checksum:i62AF611E6A615F15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25547 mRNA. Translation: AAB05992.1.
PIRiS63667.
RefSeqiNP_001235175.1. NM_001248246.1.
UniGeneiGma.33453.

Genome annotation databases

GeneIDi547855.
KEGGigmx:547855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25547 mRNA. Translation: AAB05992.1.
PIRiS63667.
RefSeqiNP_001235175.1. NM_001248246.1.
UniGeneiGma.33453.

3D structure databases

ProteinModelPortaliQ39821.
SMRiQ39821. Positions 1-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ39821. 2 interactions.
STRINGi3847.GLYMA05G36840.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi547855.
KEGGigmx:547855.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDLCA_SOYBN
AccessioniPrimary (citable) accession number: Q39821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The term phragmoplastin refers to the location of the protein to the phragmoplast across the whole width of the newly formed cell plate, unlike phragmoplast microtubules which are concentrated on the periphery of the forming plate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.