Reviewed, UniProtKB/Swiss-Prot Q397Y5 (ASPD_BURS3)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Bcep18194_B1112

Organism

Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194)) [Complete proteome] [HAMAP]

Taxonomic identifier

269483 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067300
Sites
	Active site	224	1	By similarity
	Binding site	128	1	NAD; via amide nitrogen By similarity
	Binding site	194	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q397Y5-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 22CC0523B9FBADBF	FASTA	271	27,841
	10 20 30 40 50 60 MRNAHAPVDV AMIGFGAIGA AVYHAVEHDA SLRIAHVIVP EHQCAAVQGV LGGAVEVVSS 70 80 90 100 110 120 VDALARRPEF ALECAGHSAL VDHVVPLLKA GTDCAVASIG ALSDLALLDV LAQAADEGDA 130 140 150 160 170 180 TVTLLSGAIG GIDALASAKQ GGLDEVLYVG RKPPLGWLGT PAEALCDLRA MTGEKVIFEG 190 200 210 220 230 240 SARDAARLYP KNANVAATVA LAGLGLDATH VRLIADPSVE RNVHRITARG AFGEMSLEMS 250 260 270 GKPLPDNPKT SALTAYSAIR ALRNRAARCV I

References

[1]

"Complete sequence of chromosome 2 of Burkholderia sp. 383." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000152 Genomic DNA. Translation: ABB11226.1.
RefSeq	YP_371870.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q397Y5.
Genome annotation databases
GeneID	3752877.
GenomeReviews	Gene locus Bcep18194_B1112 in contig CP000152_GR.
KEGG	bur:Bcep18194_B1112.
NMPDR	fig|269483.3.peg.1109.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q397Y5.
OMA	ECAGHSA.
Enzyme and pathway databases
BioCyc	BSP36773:BCEP18194_B1112-MON.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_BURS3

Accession

Primary (citable) accession number: Q397Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)