Q39799 (CHI1_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endochitinase 1 EC=3.2.1.14
Organism	Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifier	3635 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 317	295	Endochitinase 1		PRO_0000005292
	Propeptide	318 – 324	7	Removed in mature form By similarity		PRO_0000005293
Regions
	Domain	23 – 64	42	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	25 ↔ 40		By similarity
	Disulfide bond	34 ↔ 46		By similarity
	Disulfide bond	39 ↔ 53		By similarity
	Disulfide bond	58 ↔ 62		By similarity
	Disulfide bond	95 ↔ 158		By similarity
	Disulfide bond	170 ↔ 178		By similarity
	Disulfide bond	277 ↔ 309		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39799 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9923B123EF5C7E3E	FASTA	324	34,666
	10 20 30 40 50 60 MSFLQALSIF LLLLLYVVVG SAEQCGRQAG GALCPGGLCC SQFGWCGSTA DYCTVPGCQS 70 80 90 100 110 120 QCSGSGPAPG PGGLTNLISR ETFNQMLLHR NDGACPARGF YTYDAFIAAA RSFPAFATTG 130 140 150 160 170 180 DQATRKREIA AFLAQTSHET TGGAGWAAPD GPYAWGYCYN RELNPPSSYC ASDPNYPCAP 190 200 210 220 230 240 GKQYFGRGPM QLSWNYNYGQ CGRAIGVDLL NNPDLLSSDP TISFKSAFWF WMTPQSPKPS 250 260 270 280 290 300 CHNVIIGAWS PSSSDRAAGR VPGYGVITNI INGGLECGKG WNAQVEDRIG FYKRYCDILG 310 320 VSYGNNLDCY NQSPFGNGVS VDSM

References

[1]	"Isolation of a genomic DNA clone from Gossypium hirsutum with high similarity to class I endochitinase plant sequences." Levorson J.P., Chlan C.A. Plant Gene Register PGR96-054 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Coker 201.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60197 Genomic DNA. Translation: AAB67842.1.
PIR	T10802.
3D structure databases
ProteinModelPortal	Q39799.
SMR	Q39799. Positions 23-316.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_GOSHI

Accession

Primary (citable) accession number: Q39799

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program