Reviewed, UniProtKB/Swiss-Prot Q39786 (PGLR_GOSHI)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

Name:

G9

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function in the depolymerization of the pectin in its walls during pollen tube elongation, or in that of the pistil during pollination.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted By similarity. Secreted › cell wall By similarity.
Tissue specificity	Pollen.
Developmental stage	Appears 12 days before anthesis and maximum levels are seen in pollen on the day of anthesis.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 407	381	Polygalacturonase		PRO_0000024803
Sites
	Active site	221	1	Proton donor By similarity
	Active site	244	1	By similarity
Amino acid modifications
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39786-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B81B2BC4C312D195	FASTA	407	43,921
	10 20 30 40 50 60 MAPHLNIVPS MFVLLLLFIS ASKVQSDAFD VVAKFGAKAD GKTDLSKPFL DAWKEACASV 70 80 90 100 110 120 TPSTVVIPKG TYLLSKVNLE GPCKAPIEIN VQGTIQAPAD PSAFKDPNWV RFYSVENFKM 130 140 150 160 170 180 FGGGIFDGQG SIAYEKNTCE NREFRSKLPV NIRFDFLTNA LIQDITSKDS KLFHINVFAC 190 200 210 220 230 240 KNITLERLKI EAPDESPNTD GIHMGKSEGV NIIASDIKTG DDCISIGDGT KNMVIKEITC 250 260 270 280 290 300 GPGHGISIGS LGKFQNEEPV EGIKISNCTI TNTSNGARIK TWPGEHGGAV SEIHFEDITM 310 320 330 340 350 360 NNVSSPILID QQYCPWNKCK KNEESKVKLS NISFKNIRGT SALPEAIKFI CSGSSPCQNV 370 380 390 400 ELADIDIKHN GAEPATSQCL NVKPITSGKL NPIPCSGPVP KTPSATA

References

[1]

"Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco." John M.E., Petersen M.W. Plant Mol. Biol. 26:1989-1993(1994) [PubMed: 7858233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Coker 312. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09717 mRNA. Translation: AAA82167.1.
PIR	S52006.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 1857.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_GOSHI

Accession

Primary (citable) accession number: Q39786

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)