Q39785 (CHI2_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endochitinase 2 EC=3.2.1.14
Organism	Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifier	3635 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	302 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Endochitinase 2		PRO_0000005294
	Propeptide	296 – 302	7	Removed in mature form By similarity		PRO_0000005295
Regions
	Domain	‹1 – 42	›42	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	3 ↔ 18		By similarity
	Disulfide bond	12 ↔ 24		By similarity
	Disulfide bond	17 ↔ 31		By similarity
	Disulfide bond	36 ↔ 40		By similarity
	Disulfide bond	73 ↔ 136		By similarity
	Disulfide bond	148 ↔ 156		By similarity
	Disulfide bond	255 ↔ 287		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q39785 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: F6BD0C9AB6BC1870	FASTA	302	32,262
	10 20 30 40 50 60 EQCGRQAGGA LCPGGLCCSQ FGWCGSTADY CTVPGCQSQC SGSGPAPGPG GLTNLISRET 70 80 90 100 110 120 FNQMLLHRND GACPARGFYT YDAFIAAARS FPAFATTGDQ ATRKREIAAF LAQTSHETTG 130 140 150 160 170 180 GAGWAAPDGP YAWGYCYNRE LNPPSSYCAS DPNYPCAPGK QYFGRGPMQL SWNYNYGQCG 190 200 210 220 230 240 RAIGVDLLNN PDLLSSDPTI SFKSAFWFWM TPQSPKPSCH NVIIGAWSPS SSDRAAGRAT 250 260 270 280 290 300 GYGVITNIIN GGLECGKGWN AQVEDRIGFY KRYCDILGVS YGNNLDCYNQ SPFGNGVSVD SM

References

[1]	"Cloning of an ethylene-responsive chitinase from cotton." Levorson J.P., Chlan C.A. Plant Gene Register PGR97-034 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Stoneville 887. Tissue: Leaf.
[2]	Levorson J.P., Chlan C.A. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 112-205. Strain: cv. Stoneville 887. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78888 mRNA. Translation: AAB68047.1. U57408 Genomic DNA. Translation: AAB01339.1.
PIR	T10810.
3D structure databases
ProteinModelPortal	Q39785.
SMR	Q39785. Positions 1-294.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_GOSHI

Accession

Primary (citable) accession number: Q39785

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program