Reviewed, UniProtKB/Swiss-Prot Q39769 (G3PC_GINBI)

Names and origin

Protein names	Recommended name:     Glyceraldehyde-3-phosphate dehydrogenase, cytosolic     EC=1.2.1.12
Organism	Ginkgo biloba (Ginkgo)
Taxonomic identifier	3311 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Ginkgophyta › Ginkgoales › Ginkgoaceae › Ginkgo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145600
Regions
	Nucleotide binding	16 – 17	2	NAD By similarity
	Region	156 – 158	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	216 – 217	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	157	1	Nucleophile By similarity
	Binding site	38	1	NAD By similarity
	Binding site	85	1	NAD; via carbonyl oxygen By similarity
	Binding site	187	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	239	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	321	1	NAD By similarity
	Site	184	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39769-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D450659E369CFD7	FASTA	340	36,769
	10 20 30 40 50 60 MSSTGKIKIG INGFGRIGRL VARVALLRDD IELVAVNDPF ISTDYMTYMF KYDSVHGQWK 70 80 90 100 110 120 KHEVKVKDSN TLLFGEKAVT VFGCRNPEEI PWGETGVEYV VESTGVFTDK EKAAAHIKGG 130 140 150 160 170 180 AKKVVITAPS KDAPMFVVGV NEHEYKPDLA IVSNASCTTN CLAPLAKVIN DRFGIVEGLM 190 200 210 220 230 240 TTVHSITATQ KTVDGPSNKD WRGGRGAGFN IIPSSTGAAK AVGKVLPALN GKLTGMAFRV 250 260 270 280 290 300 PTPDVSVVDL TVRLEKPASY DEIKAAIKEE SEGKLKGILG YTEDDVVSTD FIGDNRSSIF 310 320 330 340 DAKAGIALSD NFVKLVSWYD NEWGYSSRVI DLIVHMASTV

References

[1]	"Nucleotide distribution in gymnosperm nuclear sequences suggests a model for GC-content change in land-plant nuclear genomes." Jansson S., Meyer-Gauen G., Cerff R., Martin W. J. Mol. Evol. 39:34-46(1994) [PubMed: 8064871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26924 mRNA. Translation: AAA33352.1.
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_GINBI

Accession

Primary (citable) accession number: Q39769

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)