Reviewed, UniProtKB/Swiss-Prot Q39693 (INV3_DAUCA)

Names and origin

Protein names

Recommended name:
    Beta-fructofuranosidase, insoluble isoenzyme 3
    EC=3.2.1.26
Alternative name(s):
    Sucrose hydrolase 3
    Invertase 3
    Cell wall beta-fructosidase 3

Gene names

Name:

INV3

Organism

Daucus carota (Carrot)

Taxonomic identifier

4039 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › Scandiceae › Daucinae › Daucus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May play an important role in phloem unloading and in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › cell wall. Note: Ionically bound to the cell wall.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Propeptide	34 – ?		Potential		PRO_0000033370
	Chain	? – 583		Beta-fructofuranosidase, insoluble isoenzyme 3		PRO_0000033371
Amino acid modifications
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	561	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39693-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1DBF591CD94749AF	FASTA	583	66,381
	10 20 30 40 50 60 MARTKILVFS SDSSLFLLSI FSFIFLNING VDSTHRVFPE LQSISAVDVK LVHRTGYHFQ 70 80 90 100 110 120 PQKHWINDPN GPMFYKGYYH LFYQYNPKGS VWGNIVWAHS VSKDLINWIA LEPAIFPSKP 130 140 150 160 170 180 FDQYGCWSGS ATILPGNKPV ILYTGIVSPD PENAQVQNYA VPANYSDPFL REWVKPDNNP 190 200 210 220 230 240 LVGVHTENPS AFRDPTTAWF DGGHWKMLVG SSRKHRGIAY LYRSKDFKKW KRSPHPIHTK 250 260 270 280 290 300 AETGMWECPD FYPVSPRSED GLDNSKMGRG IKHVLKVSLN STRYEYYTIG RYNRVRDFYV 310 320 330 340 350 360 PDNTSVDGWA GLRYDYGNFY ASKTFYDPIK KRRILWGWAN ESDSQIDDVQ KGWAGIQLIP 370 380 390 400 410 420 RRIWLDPSGR QLVQWPIEEV EGLRGSELHM RNQKLDMGVH VEVTGITAAQ ADVDATFSFK 430 440 450 460 470 480 SLDKAESFDP EWINLDAQDV CDSMGSTIQG GLGPFGLLTL ASKDLEEYTP VFFRIFKAED 490 500 510 520 530 540 QKLKVLMCSD AKRSSLAEGL YKPSFRGFVD VDLSDKKISL RSLIDNSVVE SFGAQRKNLI 550 560 570 580 SSRVYPTLAI YNNAHLFVFN NGTEPITVDN LDAWSMNSPS EMN

References

[1]

"Structural organization and differential expression of carrot beta-fructofuranosidase genes: identification of a gene coding for a flower bud-specific isozyme." Lorenz K., Lienhard S., Sturm A. Plant Mol. Biol. 28:189-194(1995) [PubMed: 7787183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Queen Anne's Lace.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78423 Genomic DNA. Translation: CAA55188.1.
PIR	S56680.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BRENDA	3.2.1.26. 1081.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV3_DAUCA

Accession

Primary (citable) accession number: Q39693

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)