Q39692 (INV2_DAUCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 2
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 2
Invertase 2
Sucrose hydrolase 2

Gene names

Name:

INV2

Organism

Daucus carota (Carrot)

Taxonomic identifier

4039 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiineae › Apiaceae › Apioideae › Scandiceae › Daucinae › Daucus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play an important role in phloem unloading and in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › cell wall. Note: Ionically bound to the cell wall.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Potential
	Propeptide	41 – ?		Potential		PRO_0000033368
	Chain	? – 592		Beta-fructofuranosidase, insoluble isoenzyme 2		PRO_0000033369
Sites
	Active site	75	1	By similarity
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	568	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q39692 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2734603836709133	FASTA	592	67,398
	10 20 30 40 50 60 MLIRCFHIKM ALVTCFHSML FLSAVVFIFS LDVNIRGVEA SHQVFPELQS VSAVNVQLVH 70 80 90 100 110 120 RTGYHFQPKK HWINDPNGPM YYKGFYHLFY QYNPKGAVWG NIVWAHSISK DLINWVALEP 130 140 150 160 170 180 AIFPSKPFDK YGCWSGSATV LPGGKPVIMY TGIVTPSPVN TQVQNFAVPA NYSDPYLREW 190 200 210 220 230 240 IKPDNNPIVR ARSENSSSFR DPTTAWFDGV HWKILVGSRR KHRGIAYLYR SRNFLKWTKA 250 260 270 280 290 300 KHPLHSKDRT GMWECLDFYP VAPKGMNGLD TSVTGQDIKH VLKVSLYSTR YEYYTVGEYD 310 320 330 340 350 360 RDNDIYVPDN TSVDGWAGLR YDYGNFYASK TFFDPDKQRR ILWGWANESD SKQDDVQKGW 370 380 390 400 410 420 AGIQLIPRKL WLDPNGKQLI QWPIEEIQLL RGQNVHMGSQ VLNTGEHIEV KGVTAAQADV 430 440 450 460 470 480 DATFSFKSLD RAEWFDPNWA KLDALDVCDW MGSTVRGGLG PFGFLTLASE KLEEYTPVFF 490 500 510 520 530 540 RVFKTKDKLK VLMCSDAKRS STTAEGLYKP PFAGYVDVDL SDKKISLRSL IDNSVVESFG 550 560 570 580 590 AHGRTCITSR VYPKIAIYNN AHVFVFNNGT EAITIDSLDA WSMKAPSLMN NN

References

[1]

"Structural organization and differential expression of carrot beta-fructofuranosidase genes: identification of a gene coding for a flower bud-specific isozyme." Lorenz K., Lienhard S., Sturm A. Plant Mol. Biol. 28:189-194(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Queen Anne's Lace.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78424 Genomic DNA. Translation: CAA55189.1.
PIR	S56681.
3D structure databases
ProteinModelPortal	Q39692.
SMR	Q39692. Positions 59-587.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV2_DAUCA

Accession

Primary (citable) accession number: Q39692

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program