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Q39659

- MFPA_CUCSA

UniProt

Q39659 - MFPA_CUCSA

Protein

Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a

Gene
N/A
Organism
Cucumis sativus (Cucumber)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.1 Publication
    (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.1 Publication
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.1 Publication
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
    2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
    3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
    4. coenzyme binding Source: InterPro
    5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
    6. enoyl-CoA hydratase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
    3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
    OrganismiCucumis sativus (Cucumber)Imported
    Taxonomic identifieri3659 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

    Subcellular locationi

    Glyoxysome 1 Publication

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-aPRO_0000109251Add
    BLAST

    Proteomic databases

    PRIDEiQ39659.

    Structurei

    3D structure databases

    ProteinModelPortaliQ39659.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.1 Publication

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

    Phylogenomic databases

    KOiK10527.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q39659-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSNAKGRTV MEVGTDGVAI ITIINPPVNS LSFDVLFSLR DSYEQALRRD    50
    DVKAIVVTGA KGKFSGGFDI TAFGVLQGGK GEQPNVRNIS IEMITDIFEA 100
    ARKPAVAAID GLALGGGLEV AMACHARIST PTAQLGLPEL QLGIIPGFGG 150
    TQRLPRLVGL SKALEMMLTS KPIKGQEAHS LGLVDAIVPP EELINTARRW 200
    ALEILERRRP WVHSLHRTDK LESLAEARKI FNLARAQAKK QYPNLKHTIA 250
    CIDAVETGVV SGPRAGLWKE AEEFQGLLHS DTCKSLIHIF FAQRSTTKVP 300
    GVTDLGLVPR QIKKVAIVGG GLMGSGIATA LILSNYHVVL KEVNDKFLQA 350
    GIDRVRANLQ SRVKKGNMTN EKFEKSISLL KGVLNYESFK DVDMVIEAVI 400
    ENVSLKQQIF SDLEKYCPPH CMLATNTSTI DLELIGERIK SRDRIIGAHF 450
    FSPAHIMPLL EIVRTKHTAA QVIVDLLDVG KNIKKTPVVV GNCTGFAVNR 500
    MFFPYSQAAI LLAEHGVDPY QIDRAISKFG MPMGPFRLCD LVGFGVAAAT 550
    ASQFVQAFPE RTYKSMLIPL MQEDKNAGES TRKGFYVYDK NRKAGPNPEL 600
    KKYIEKARNS SGVSVDPKLT KLPEKDIVEM IFFPVVNEAC RVLAEGIAVK 650
    AADLDIAGVM GMGFPSYRGG LMFWADSLGS NYIYSRLEEW SKQYGGFFKP 700
    CGYLAERAVQ GATLSAPGGH AKPRM 725
    Length:725
    Mass (Da):79,170
    Last modified:November 1, 1996 - v1
    Checksum:iD7C7943A51AFE7DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78996 mRNA. Translation: CAA55630.1.
    PIRiT10464.
    RefSeqiXP_004168411.1. XM_004168363.1.

    Genome annotation databases

    GeneIDi101210295.
    KEGGicsv:101210295.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78996 mRNA. Translation: CAA55630.1 .
    PIRi T10464.
    RefSeqi XP_004168411.1. XM_004168363.1.

    3D structure databases

    ProteinModelPortali Q39659.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q39659.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101210295.
    KEGGi csv:101210295.

    Phylogenomic databases

    KOi K10527.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Domains of the tetrafunctional protein acting in glyoxysomal fatty acid beta oxidation. Demonstration of epimerase and isomerase activities on a peptide lacking hydratase activity."
      Preisig-Mueller R., Guehnemann-Schaefer K., Kindl H.
      J. Biol. Chem. 269:20475-20481(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
      Tissue: Seedling cotyledon.

    Entry informationi

    Entry nameiMFPA_CUCSA
    AccessioniPrimary (citable) accession number: Q39659
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3