Q39639 (PLSB_CUCSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glycerol-3-phosphate acyltransferase, chloroplastic Short name=GPAT EC=2.3.1.15
Organism	Cucumis sativus (Cucumber)
Taxonomic identifier	3659 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Benincaseae › Cucumis

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Plastid › chloroplast stroma.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 101	101	Chloroplast By similarity
	Chain	102 – 470	369	Glycerol-3-phosphate acyltransferase, chloroplastic		PRO_0000024697
Regions
	Motif	240 – 245	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q39639 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AF60144E19D2B39B	FASTA	470	51,876
	10 20 30 40 50 60 MFILSAVSSS SSSSSSVPSS LPPFSLSPSI SLSFSRVSLP PSSSSSSSSL KLFLPLSLHF 70 80 90 100 110 120 TPPKLSSPHS FLRFSASRAM AELIQDKESA HTPSTTDVTR NDPPHSRAFL DLRSEEELLS 130 140 150 160 170 180 CIRRETEAGK LPSNVAAGME ELYQNYKNAV FESGNPKADE IVLSNMTVAL DRILLDVEDP 190 200 210 220 230 240 FMFSPHHKAI REPFDYYTFG QNYVRPLIDF ENSFVGNLSL FKDIEEKLHQ GHNVVLISNH 250 260 270 280 290 300 QTEADPAIIS LLLEKTNPYI AENMIYVAGD RVIADPLCKP FSIGRNLICV YSKKHMLDIP 310 320 330 340 350 360 ELAETKRKAN TRSLKEMALL LRGGSQLIWI APSGGRDRPD PSTGEWYPAP FDASSVDNMR 370 380 390 400 410 420 RLLQHSGAPG HLYPLALLCY DIMPPPSQVE IEIGEKRVIS FNGTGLSVGP EISFDEIAAS 430 440 450 460 470 RDNPDEVREA YSKALYDSVA KQYNVLKAAI DGKQELEASV ADVSLSQPWI

References

[1]	"Nucleotide sequence of acyl-acyl carrier protein: glycerol-3-phosphate acyltransferase from cucumber." Johnson T.C., Schneider J.C., Somerville C. Plant Physiol. 99:771-772(1992) [PubMed: 16668954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80571 mRNA. Translation: AAA33122.1.
PIR	T10193.
3D structure databases
ProteinModelPortal	Q39639.
SMR	Q39639. Positions 105-469.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR016222. G3P_O-acylTrfase_chlp. IPR023083. G3P_O-acylTrfase_N. [Graphical view]
Gene3D	G3DSA:1.10.1200.50. G3P_O-acylTrfase_N. 1 hit.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000431. Glycerol-3-P_O-acyltransfrase. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_CUCSA

Accession

Primary (citable) accession number: Q39639

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program