Q39586 (METE_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase EC=2.1.1.14 Alternative name(s): Cobalamin-independent methionine synthase Methionine synthase, vitamin-B12 independent isozyme
Organism	Chlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier	3055 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	814 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Zinc By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 814	814	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase		PRO_0000098701
Sites
	Metal binding	655	1	Zinc By similarity
	Metal binding	657	1	Zinc By similarity
	Metal binding	744	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q39586 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D6704D1C446EA72	FASTA	814	86,502
	10 20 30 40 50 60 MLSTTTIGFP RIGNQRQLKF AMESYFKGDS GEAELLAVAH KVQSDAWALQ KAAGIAVIGL 70 80 90 100 110 120 DGTLYDQVLD TITWLGAIPP RFKHLSGLQR YYAMARGGAA LDMSKFFDTN YHYLVPELGP 130 140 150 160 170 180 DVLGPATAGP PLQPDFSGPL DKLARGQAVV GRDGAVPILI GPVTFVSLSR GCELPLDQAV 190 200 210 220 230 240 ARLLPTYCAL LQQLAAAGAP EVQLHEPVLA TSEGTGMPTE FETAYAQMAQ AAGSVPLHLV 250 260 270 280 290 300 TYYDDLGAAY PWAVQLPVAA VTLDFLGPPG AAVPSQTLAL LQQHGFPADK RLGAGVVDGR 310 320 330 340 350 360 SVWKDDGTAV ALLRALLDTG AVSSDRLVVT SSAPLQHLPY DLGLELEAPK TPEATAPAAR 370 380 390 400 410 420 AGRPPGFAKQ KVEEIVSVAR LAASPQPPLL QLVMAPWCSC SGCSRARAWR TTPPTSRLSG 430 440 450 460 470 480 SAAKPYDVRA RDEQLQLAAF PTTTIGSFPQ TAEVRRLRQQ LKSGRLTQAE YEGLIAGHIA 490 500 510 520 530 540 HAVGVQEALG IDVLVHGEAE RTDMVEYFGM QLGGMLFTRA GWVQSYGSRC VRPPLVVDDI 550 560 570 580 590 600 TYRGPSTCWE YKVAIPTAAQ PVKGLLTGPV TILNWSFPRK DISRAAQAMQ LGLALRQEVA 610 620 630 640 650 660 ALEAAGCTII QVDDPALREG LPLNGERWAS YLSWAVDAFR LCTGVAAAGT QVVTHLCYSD 670 680 690 700 710 720 FQDILPAIDR MDADVLTIEN SRSDKPMMAA LAAAGYGRDI GPGVYDVHSP VVPSVEFIKS 730 740 750 760 770 780 RIRSFVDSGI LSGRYDRIWV NPDCGLKTRG WPETIAALAN MVEAAAQARA ELQLAGGAAV 790 800 810 APVTGGVEAA GKGGAGQCRR NQPLRGLLPL SCLP

References

[1]	"Increased transcript levels of a methionine synthase during adhesion-induced activation of Chlamydomonas reinhardtii gametes." Kurvari V., Qian F., Snell W.J. Plant Mol. Biol. 29:1235-1252(1995) [PubMed: 8616221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 21gr / CC-1690.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36197 mRNA. Translation: AAC49178.1.
PIR	S65083.
3D structure databases
ProteinModelPortal	Q39586.
ModBase	Search...
Protein-protein interaction databases
STRING	Q39586.
Proteomic databases
PRIDE	Q39586.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CHLRE

Accession

Primary (citable) accession number: Q39586

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program